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Dynamic Monitoring of Seroconversion using a Multianalyte Immunobead Assay for Covid-19

Dynamic Monitoring of Seroconversion using a Multianalyte Immunobead Assay for Covid-19

Published on: February 16, 2022

Comparative study of different SARS-CoV-2 diagnostic techniques.

L Vallejo¹, M Martínez-Rodríguez¹, M J Nieto-Bazán¹

¹Department of Clinical Microbiology, IML and IdISSC, Hospital Clínico San Carlos, Madrid, Spain.

Journal of Virological Methods

|September 16, 2021

Summary

This summary is machine-generated.

Four commercial RT-qPCR kits and two other systems were evaluated for detecting SARS-CoV-2. All tested methods showed value for virus detection, with qCOVID19 and Allplex™SARS-COV-2 Assay detecting all clinical samples.

Keywords:

Microarrays RT-qPCR SARS-CoV-2 detection TMA

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Area of Science:

Virology
Molecular Diagnostics
Infectious Disease Research

Background:

The rapid global spread of SARS-CoV-2 necessitated the development of accurate and swift diagnostic tests.
Numerous commercial platforms for rapid virus detection have emerged and received approval.

Purpose of the Study:

To evaluate the performance of four commercial RT-qPCR kits and two other diagnostic systems for SARS-CoV-2 detection.
To compare the sensitivity and reliability of different commercial assays using clinical samples and serial dilutions.

Main Methods:

Analysis of 95 positive and 5 negative retrospective clinical samples.
Testing of four RT-qPCR kits: TaqMan 2019nCoV Assay, Allplex™SARS-COV-2 Assay, FTD SARS-COV-2 Assay, and qCOVID-19.
Evaluation of Hologic Aptima SARS-COV-2 and Clart-COVID-19 systems, including sensitivity analysis with SARS-CoV-2 standard dilutions.

Main Results:

qCOVID-19 and Allplex™SARS-COV-2 Assay detected all clinical samples.
All four RT-qPCR kits demonstrated high sensitivity for samples with Ct<33.
Clart-COVID-19 microarrays successfully detected all samples and controls, while Hologic Aptima Panther failed with one sample and had numerous invalidated results.

Conclusions:

All evaluated diagnostic techniques are valuable for SARS-CoV-2 detection.
Specific RT-qPCR assays like qCOVID-19 and Allplex™SARS-COV-2 Assay showed excellent performance in detecting clinical samples.
The Clart-COVID-19 system demonstrated reliable detection, whereas the Hologic Aptima Panther system presented challenges with sample validation.