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Breaking bad: Better call gingerol for improving antibiotic susceptibility of Pseudomonas aeruginosa by inhibiting

Arpit Shukla1, Paritosh Parmar2, Baldev Patel2

  • 1Department of Microbiology & Biotechnology, University School of Sciences, Gujarat University, Ahmedabad, 380009, Gujarat, India; Department of Biological Sciences & Biotechnology, Institute of Advanced Research, Gandhinagar, 382426, Gujarat, India.

Microbiological Research
|September 16, 2021
PubMed

Insights

Gingerol inhibits quorum sensing (QS) pathways in Pseudomonas aeruginosa, a multi-antibiotic resistant

Area of Science:

  • Microbiology
  • Bacteriology
  • Drug Discovery

Background:

  • Pseudomonas aeruginosa is a multidrug-resistant 'superbug' causing significant infections.
  • Bacterial pathogenicity is often regulated by quorum sensing (QS), a cell-cell communication system.
  • QS pathways are crucial for P. aeruginosa virulence and pose challenges for antibiotic treatment.

Purpose of the Study:

  • To investigate gingerol as a potential quorum sensing inhibitor (QSI) against P. aeruginosa.
  • To evaluate gingerol's efficacy in disrupting QS-mediated virulence factors.
  • To assess the combined effect of gingerol and antibiotics against P. aeruginosa.

Main Methods:

  • In silico analysis to predict gingerol's interaction with QS receptors (LasR, PhzR, RhlR).
  • In vitro validation of gingerol's impact on EPS, biofilm, pyocyanin, and rhamnolipid production.
  • Assessment of gingerol's synergistic effect with ciprofloxacin on bacterial growth.

Main Results:

  • Gingerol effectively interfered with key QS receptors in P. aeruginosa.
  • Gingerol significantly reduced virulence factors including EPS, biofilm, pyocyanin, and rhamnolipid.
  • Gingerol enhanced the antibacterial activity of ciprofloxacin, demonstrating synergistic effects.

Conclusions:

  • Gingerol shows promise as a novel quorum sensing inhibitor against P. aeruginosa.
  • Combining QSIs like gingerol with conventional antibiotics offers a potent strategy against antibiotic resistance.
  • This integrated approach could provide a holistic solution for combating multidrug-resistant bacterial infections.
Keywords:
Antibiotic sensitivityBiofilmComputational biologyMolecular dynamicsPhenazineRhamnolipid

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