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A simple fluorometric method to discriminate adriamycin-resistant subline from adriamycin-sensitive parental P388
K Dekker1, N Kojima, S Yamamoto
1Institute of Applied Biochemistry, Yagi Memorial Park, Gifu, Japan.
Biochemistry International
|June 1, 1987
Abstract:
A fluorometric method to estimate adriamycin accumulation in cells was used to discriminate adriamycin- and vincristine-resistant sublines from sensitive parental P388 murine leukemia cells. It was easily checked that adriamycin accumulation was lower in the adriamycin- and vincristine-resistant cells than in the sensitive ones. With this method we found that tamoxifen as well as diltiazem increased adriamycin accumulation in the resistant cells to a level similar to that in the sensitive ones. The effect of progesterone was much less.