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TFF-1 Functions to Suppress Multiple Phenotypes Associated with Lung Cancer Progression
Kentaro Minegishi1, Yoh Dobashi2,3, Hiroyoshi Tsubochi1
1Department of Thoracic Surgery, Saitama Medical Center, Jichi Medical University, Saitama, Japan.
Oncotargets and Therapy
|September 17, 2021
Summary
Trefoil Factor-1 (TFF-1) suppresses lung carcinoma cell proliferation, migration, and invasion by inducing apoptosis. This protein may offer a novel therapeutic target for lung cancer treatment.
Area of Science:
- Oncology
- Molecular Biology
- Biochemistry
Background:
- Trefoil Factor (TFF) proteins, including TFF-1, have dual roles in cancer, potentially promoting or suppressing tumor progression.
- The specific function of TFF-1 in lung carcinoma remained largely undefined prior to this study.
Purpose of the Study:
- To elucidate the pathobiological role of TFF-1 in human lung carcinoma.
- To investigate the effects of TFF-1 on cancer cell phenotypes, including proliferation, apoptosis, migration, and invasion.
Main Methods:
- Examined TFF-1 expression and secretion in lung carcinoma cell lines using immunoblotting, immunofluorescence, ELISA, and qRT-PCR.
- Analyzed the impact of TFF-1 overexpression on cell proliferation, death (apoptosis), cell cycle, migration, and invasion.
- Utilized TFF-1-specific siRNA to confirm the suppressive effects of TFF-1.
Main Results:
- Endogenous TFF-1 was expressed and secreted in adenocarcinoma cell lines.
- TFF-1 overexpression decreased proliferation by 19-25% via apoptosis induction (caspase 3/7 activation).
- TFF-1 suppressed cell migration (71-82%) and invasion (77-83%), effects reversible by TFF-1 siRNA.
Conclusions:
- TFF-1 acts as a tumor suppressor in lung carcinoma by inhibiting proliferation, migration, and invasion through apoptosis induction.
- TFF-1 represents a potential synergistic therapeutic target for human lung carcinoma treatment strategies.
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