Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Matrix Stiffness Induces Endothelial Network Senescence.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

Src and β-catenin control a senescence-fibrotic phenotype in uterine fibroid cells.

Scientific reports·2026
Same author

Distinct fibroblast and perivascular senotypes define spatial niches that regulate fibrosis.

bioRxiv : the preprint server for biology·2026
Same author

γδ T cell-stromal networks modulate matrix composition and vascularity in foreign body response.

Nature communications·2026
Same author

Protein-Linker Co-engineering for Broad-Spectrum Antiviral Development against Enveloped Viruses.

ACS materials letters·2026
Same author

Direct Measurement of Protein Pair Interaction Potential.

ACS nano·2026
Same journal

Shear-Induced CROSS (Cellular RedOx Spreading Shield) Assembly Sustains Neurotrophic Extracellular Vesicle Production for Functional Neural Networks.

Advanced functional materials·2026
Same journal

Buckling-Resistant and Trace-Stacked (BRATS) Design Enables Aid-Free Implantation of Flexible Multielectrode Array with Minimized Inflammatory Tissue Response.

Advanced functional materials·2026
Same journal

Rationally designed anisotropic and auxetic hydrogel patches for adaptation to dynamic organs.

Advanced functional materials·2026
Same journal

Benchtop Fabrication and Integration of Laser-Induced Graphene Strain Gauges and Stimulation Electrodes in Muscle on a Chip Devices.

Advanced functional materials·2026
Same journal

Controlling 3D Contractility via Engineered Fibrous Hydrogel Composites.

Advanced functional materials·2026
Same journal

Cardiac-Derived ECM Microspheres for Enhanced hiPSC-CMs Maturation.

Advanced functional materials·2026
See all related articles

Related Experiment Video

Updated: Oct 20, 2025

Combination of Microstereolithography and Electrospinning to Produce Membranes Equipped with Niches for Corneal Regeneration
11:42

Combination of Microstereolithography and Electrospinning to Produce Membranes Equipped with Niches for Corneal Regeneration

Published on: September 12, 2014

12.6K

Cyclodextrin Modulated Type I Collagen Self-Assembly to Engineer Biomimetic Cornea Implants.

Shoumyo Majumdar1, Xiaokun Wang1, Sven D Sommerfeld1

  • 1Translational Tissue Engineering Center, Johns Hopkins University, Baltimore, MD 21231, USA.

Advanced Functional Materials
|September 17, 2021
PubMed
Summary
This summary is machine-generated.

Cyclodextrins (CDs) regulate collagen self-assembly, creating robust, transparent biosynthetic corneal materials. These engineered implants integrate into rabbit corneas, showing potential for tissue reconstruction.

Keywords:
collagencorneacyclodextrinsfibril alignmentself-assembly

More Related Videos

Three-dimensional Biomimetic Technology: Novel Biorubber Creates Defined Micro- and Macro-scale Architectures in Collagen Hydrogels
12:07

Three-dimensional Biomimetic Technology: Novel Biorubber Creates Defined Micro- and Macro-scale Architectures in Collagen Hydrogels

Published on: February 12, 2016

9.4K
Engineering 3D Cellularized Collagen Gels for Vascular Tissue Regeneration
09:23

Engineering 3D Cellularized Collagen Gels for Vascular Tissue Regeneration

Published on: June 16, 2015

21.2K

Related Experiment Videos

Last Updated: Oct 20, 2025

Combination of Microstereolithography and Electrospinning to Produce Membranes Equipped with Niches for Corneal Regeneration
11:42

Combination of Microstereolithography and Electrospinning to Produce Membranes Equipped with Niches for Corneal Regeneration

Published on: September 12, 2014

12.6K
Three-dimensional Biomimetic Technology: Novel Biorubber Creates Defined Micro- and Macro-scale Architectures in Collagen Hydrogels
12:07

Three-dimensional Biomimetic Technology: Novel Biorubber Creates Defined Micro- and Macro-scale Architectures in Collagen Hydrogels

Published on: February 12, 2016

9.4K
Engineering 3D Cellularized Collagen Gels for Vascular Tissue Regeneration
09:23

Engineering 3D Cellularized Collagen Gels for Vascular Tissue Regeneration

Published on: June 16, 2015

21.2K

Area of Science:

  • Biomaterials Science
  • Ophthalmology
  • Tissue Engineering

Background:

  • Corneal extracellular matrix (ECM) ultrastructure is crucial for optical transparency and mechanical strength.
  • Small leucine-rich proteoglycans (SLRPs) regulate corneal collagen fibril organization during development.
  • Mimicking native cornea structure requires precise control over collagen assembly.

Purpose of the Study:

  • To investigate cyclodextrins (CDs) for regulating collagen assembly in biosynthetic materials.
  • To engineer corneal substitutes with native-like structural and optical properties.
  • To evaluate the in vivo performance of CD-modified collagen implants for corneal reconstruction.

Main Methods:

  • Screening of various cyclodextrins (CDs) for their ability to regulate collagen assembly during vitrification.
  • Characterization of self-assembled collagen-vitrigel materials with beta-cyclodextrin (βCD).
  • Engineering of custom molds for fabricating corneal implants and in vivo implantation in a rabbit partial keratoplasty model.

Main Results:

  • Addition of βCD to collagen vitrigels yielded materials with aligned fibers and lamellae, mimicking native cornea.
  • βCD interacted with hydrophobic amino acids in collagen, influencing fibril organization and resulting in mechanically robust and transparent materials.
  • Acellular βCD/Coll implants demonstrated successful tissue integration and supported re-epithelialization in a rabbit model.

Conclusions:

  • Cyclodextrin molecules effectively regulate collagen self-assembly for creating advanced corneal mimetic substitutes.
  • The process offers a simple method for engineering biosynthetic materials with improved structural and functional properties.
  • CD-modified collagen implants show promise for corneal reconstruction applications.