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Hyperoside suppresses BMP-7-dependent PI3K/AKT pathway in human hepatocellular carcinoma cells
Shuang Wei1, Yun Sun1, Li Wang1
1Department of Rehabilitation Medicine, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China.
Background:
New therapeutics for hepatocellular carcinoma (HCC) are urgently needed and searching for new anti-cancer compounds in plant medicines may represent a promising approach. The present study was conducted to clarify the role of hyperoside (HP) and its underlying molecular mechanism in a cancer cell.
Methods:
Bone morphogenetic protein 7 (BMP-7) protein expression was measure in Human HCC tissue. In in vitro experiments, HP effects on cell proliferation and the mechanism were investigated deeply.
Results:
The result showed a higher expression of BMP-7 in human HCC compared to adjacent noncancerous counterparts, and that silencing of BMP-7 suppressed HepG2 cell proliferation, suggesting BMP-7 plays an anti-cancer role in HCC. Furthermore, we found that HP could induce cell cycle arrest in proliferating HepG2 cells at the G1 phase by decreasing BMP-7 expression and that the phosphorylation of AKT and expression of PI3K were significantly down-regulated upon treatment of HP or BMP-7 knockdown. In addition, silencing of BMP-7 abrogated the difference of AKT phosphorylation between cells with and without HP treatment.
Conclusions:
Our results indicated that HP suppressed cell proliferation by inhibiting the BMP-7-dependent PI3K/AKT signaling pathway in HepG2 HCC cells, and either HP supplement or targeting BMP-7 might be a promising treatment against HCC.
Insights
Hyperoside (HP) inhibits hepatocellular carcinoma (HCC) cell growth by decreasing Bone Morphogenetic Protein 7 (BMP-7) expression. This natural compound targets the BMP-7-dependent PI3K/AKT pathway, offering a potential new therapy for HCC.
Area of Science:
- Oncology
- Pharmacology
- Molecular Biology
Background:
- Hepatocellular carcinoma (HCC) necessitates novel therapeutic strategies.
- Plant-derived compounds offer a promising avenue for discovering new anti-cancer agents.
Purpose of the Study:
- To investigate the anti-cancer effects of hyperoside (HP) on hepatocellular carcinoma (HCC).
- To elucidate the molecular mechanisms underlying HP's action in HCC cells.
Main Methods:
- Measured Bone Morphogenetic Protein 7 (BMP-7) expression in human HCC tissues.
- Conducted in vitro experiments to assess HP's impact on HepG2 cell proliferation and signaling pathways.
Main Results:
- BMP-7 expression was elevated in HCC tissues and its silencing inhibited HepG2 cell proliferation.
- HP induced G1 cell cycle arrest in HepG2 cells by reducing BMP-7 expression.
- HP treatment or BMP-7 knockdown downregulated PI3K/AKT signaling pathway components.
Conclusions:
- HP suppresses HCC cell proliferation via inhibition of the BMP-7-dependent PI3K/AKT pathway.
- Hyperoside and targeting BMP-7 represent potential therapeutic strategies for HCC.
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