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Field Inoculation and Classification of Maize Ear Rot Caused by Fusarium verticillioides.
Chaopei Dong1, Yabin Wu1, Jingyang Gao1
1National Key Lab. of Wheat and Maize Crop Science, Collaborative Innovation Center of Henan Grain Crops, College of Agronomy, Henan Agricultural University, Zhengzhou, Henan, China.
Bio-Protocol
|September 17, 2021
Summary
Maize ear rot, a fungal disease, can be evaluated 30 days after inoculating plants with Fusarium verticillioides. This research details a method for assessing Fusarium resistance in maize.
Area of Science:
- Plant Pathology
- Agricultural Science
- Mycology
Background:
- Maize ear rot is a significant global fungal disease.
- It is primarily caused by Fusarium verticillioides and Fusarium graminearum.
Purpose of the Study:
- To establish an optimal timeframe for evaluating maize resistance to Fusarium ear rot.
- To detail a method for inoculating maize to induce ear rot for resistance assessment.
Main Methods:
- Maize was inoculated with Fusarium verticillioides at the filling stage (15 days post-pollination).
- A spore suspension (2 ml, 5 x 10^6/ml) was injected into the central part of the top ear using the pricking ear method.
Main Results:
- Phenotypic evaluation of Fusarium resistance in maize is most effective 30 days post-inoculation.
- The pricking ear method effectively induced maize ear rot for disease assessment.
Conclusions:
- Thirty days after inoculation provides a suitable window for assessing maize's phenotypic resistance to Fusarium ear rot.
- This study provides a reproducible method for inducing and evaluating maize ear rot.
