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Updated: Oct 19, 2025

The Soft Agar Colony Formation Assay
Published on: October 27, 2014
Overexpression of RNF126 is associated with poor prognosis and contributes to the progression of lung adenocarcinoma
Zhaona Sun1, Xin Liu2, Meiyuan Chen3
1Department of Cardiology, Yidu Central Hospital of Weifang, Weifang, 262500, China.
Abstract:
Aim: To document the expression and function of RNF126 in lung adenocarcinoma (LAD). Materials & methods: A total of 102 LAD patients were enrolled in this retrospective study. The mRNA and protein levels of RNF126 were tested via RT-qPCR and immunohistochemical staining, respectively. Univariate and multivariate analyses were conducted to exploring the clinical significance of RNF126 in the prognosis. Knockdown and overexpression strategies were used to validate the tumor-related roles of RNF126 both in vitro and in vivo. Results: RNF126 was highly expressed and was an independent predictor of a poor prognosis for LAD patients. We also revealed that RNF126 knockdown suppressed proliferation of LAD cells and xenografts. Conclusion: RNF126 is a potential survival predictor and therapeutic target for LAD.
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