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Updated: Oct 19, 2025
Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry
Published on: June 21, 2018
Macrophage migration inhibitory factor gene polymorphisms (SNP -173 G>C and STR-794 CATT5-8) confer risk of plaque
Jorge Hernández-Bello1, Miroslaba Rodríguez-Puente2, Jorge Gutiérrez-Cuevas3
1Instituto de Investigación en Ciencias Biomédicas, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Guadalajara, Jalisco, Mexico.
Background:
Macrophage inhibitory factor (MIF) is a pro-inflammatory cytokine secreted by several cells, including those in the immune system and the skin. The MIF gene contains the SNP -173 G> C and STR -794 CATT5-8 polymorphisms in the promoter region capable of affecting its activity. Our objective was to investigate the MIF polymorphisms as a risk factor for plaque psoriasis (PP) in the Mexican population.
Methods:
We genotyped both MIF polymorphism (rs5844572 and rs755622) in 224 PP patients with a clinical and histopathological diagnosis and 232 control subjects (CS) by the PCR-RFLP method. MIF serum levels were determined by an ELISA kit.
Results:
We found significant differences in the genotypic and allelic frequencies for the MIF -173 G>C polymorphism; carriers of the GC genotype (OR 1.51, 95% CI 1.026-2.228, p = 0.03) and the C allele (OR 1.34, 95% CI 1.005-1.807, p = 0.04) had higher odds to present with PP. Moreover, the 6C haplotype was associated with PP risk (OR 2.10, 95% CI 1.22-3.69, p < 0.01). Also, the -173 CC genotype was associated with high MIF serum levels (p < 0.05).
Conclusions:
The -173 GC genotype and the 6C haplotype of the MIF polymorphisms are associated with susceptibility to PP in the Mexican population.
Insights
Genetic variations in the macrophage inhibitory factor (MIF) gene, specifically the -173 G>C polymorphism and the 6C haplotype, increase the risk of developing plaque psoriasis (PP) in the Mexican population.
Area of Science:
- Genetics
- Immunology
- Dermatology
Background:
- Macrophage inhibitory factor (MIF) is a pro-inflammatory cytokine implicated in immune responses and skin conditions.
- The MIF gene's promoter region contains polymorphisms, including SNP -173 G>C and STR -794 CATT5-8, which can influence its activity.
- Understanding these genetic variations may reveal insights into disease susceptibility.
Purpose of the Study:
- To investigate the association between MIF gene polymorphisms (rs5844572 and rs755622) and plaque psoriasis (PP) risk in the Mexican population.
- To determine if specific MIF genotypes or haplotypes correlate with PP susceptibility.
- To explore the relationship between MIF genotypes and serum MIF levels.
Main Methods:
- Genotyping of MIF polymorphisms (rs5844572 and rs755622) using PCR-RFLP in 224 PP patients and 232 controls.
- Clinical and histopathological diagnosis for patient classification.
- Quantification of serum MIF levels via ELISA.
Main Results:
- Significant differences in genotypic and allelic frequencies for the MIF -173 G>C polymorphism were observed.
- Carriers of the -173 GC genotype (OR 1.51) and the C allele (OR 1.34) showed increased odds of having PP.
- The 6C haplotype was strongly associated with PP risk (OR 2.10), and the -173 CC genotype correlated with higher MIF serum levels.
Conclusions:
- The -173 GC genotype and the 6C haplotype of MIF polymorphisms are linked to increased susceptibility to plaque psoriasis in the Mexican population.
- These findings highlight the role of MIF genetic variations in PP pathogenesis.
- Further research could explore therapeutic strategies targeting MIF.
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