Related Experiment Video

Updated: Oct 19, 2025

Use of Viral Entry Assays and Molecular Docking Analysis for the Identification of Antiviral Candidates against Coxsackievirus A16
06:03

Use of Viral Entry Assays and Molecular Docking Analysis for the Identification of Antiviral Candidates against Coxsackievirus A16

Published on: July 15, 2019

8.1K

Cyclophilin A: a possible host modulator in Chandipura virus infection

Daya V Pavitrakar1, Nitin M Atre1, Anuradha S Tripathy1

  • 1ICMR-National Institute of Virology, 130/1 Sus Road, Pashan, Pune, 411021, India.

Archives of Virology
|September 17, 2021
PubMed

Insights

Chandipura virus (CHPV) causes severe pediatric encephalitis. Inhibiting cyclophilin A (CypA) with cyclosporin A significantly reduced CHPV replication, suggesting CypA as a therapeutic target for CHPV infection.

Area of Science:

  • Virology
  • Molecular Biology
  • Immunology

Background:

  • Chandipura virus (CHPV) is a significant cause of pediatric encephalitis in India with high mortality.
  • No vaccines or therapeutics are currently available for CHPV infection, necessitating novel treatment strategies.

Purpose of the Study:

  • To investigate the potential of targeting host proteins for CHPV antiviral therapy.
  • To evaluate the effect of inhibiting cyclophilin A (CypA) on CHPV replication.

Main Methods:

  • In vitro inhibition of CypA using cyclosporin A.
  • Quantification of CHPV titer and CypA levels.
  • In silico molecular docking and dynamics simulations of CHPV nucleoprotein and human CypA interaction.

Main Results:

  • Cyclosporin A treatment resulted in a 3-log reduction in CHPV titer.
  • CypA levels were undetectable after treatment.
  • In silico analysis confirmed stable interactions between CHPV nucleoprotein and human CypA.

Conclusions:

  • Cyclophilin A plays a role in facilitating CHPV replication.
  • CypA is a potential host factor for future antiviral drug development against CHPV.

More Related Videos

Production of Pseudotyped Particles to Study Highly Pathogenic Coronaviruses in a Biosafety Level 2 Setting08:40

Production of Pseudotyped Particles to Study Highly Pathogenic Coronaviruses in a Biosafety Level 2 Setting

Published on: March 1, 2019

59.3K
Two Methods of Heterokaryon Formation to Discover HCV Restriction Factors16:49

Two Methods of Heterokaryon Formation to Discover HCV Restriction Factors

Published on: July 16, 2012

11.8K

Related Concept Videos

Anaphase Promoting Complex00:50

Anaphase Promoting Complex

The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...
3.0K
GPCRs Regulate Adenylyl Cylase Activity01:09

GPCRs Regulate Adenylyl Cylase Activity

Some GPCRs transmit signals through adenylyl cyclase (AC), a transmembrane enzyme. AC helps synthesize second messenger cyclic adenosine monophosphate (cAMP). AC catalyzes cyclization reaction and converts ATP to cAMP by releasing a pyrophosphate. The pyrophosphate is further hydrolyzed to phosphate by the enzyme pyrophosphatase, which drives cAMP synthesis to completion. However, cAMP is rapidly degraded to 5′ AMP by the enzymes phosphodiesterase (PDE), preventing overstimulation of...
6.1K
Intracellular Movement of Viruses and Bacteria01:10

Intracellular Movement of Viruses and Bacteria

Intracellular bacteria and viruses often comprise a group of highly infectious pathogens that can cause several diseases. Bacterial pathogens include those belonging to the genus Rickettsia responsible for conditions such as rocky mountain spotted fever and the Mediterranean spotted fever; Chlamydia, a genus responsible for a sexually transmitted disease; Coxiella burnetii, an agent responsible for Q fever. Viral pathogens include vaccinia—a poxvirus, and herpes simplex virus—a...
3.0K
Positive Regulator Molecules02:39

Positive Regulator Molecules

Mitotic cell division results in daughter cells that exactly resemble the parent cell. However, errors in the DNA replication or distribution of genetic material may lead to genetic mutations that may be passed down to every new cell formed from the resulting abnormal cell. Propagation of such mutant cells is restricted through checkpoint mechanisms present at different stages of the cell cycle. These checkpoints involve regulator molecules that either promote or demote cell cycle events.
5.8K
Lytic Cycle of Bacteriophages01:30

Lytic Cycle of Bacteriophages

Bacteriophages, also known as phages, are specialized viruses that infect bacteria. A key characteristic of phages is their distinctive “head-tail” morphology. A phage begins the infection process (i.e., lytic cycle) by attaching to the outside of a bacterial cell. Attachment is accomplished via proteins in the phage tail that bind to specific receptor proteins on the outer surface of the bacterium. The tail injects the phage’s DNA genome into the bacterial cytoplasm. In the...
73.2K
Subviral Agents01:29

Subviral Agents

Subviral agents are infectious entities that resemble viruses but lack one or more viral components, such as a capsid or essential replication machinery. These agents include viroids, prions, and satellites, each possessing distinct structural and functional characteristics that influence their mode of infection and replication.Viroids are the simplest subviral agents, consisting of circular, single-stranded RNA molecules without a protein coat. They exclusively infect plants, relying entirely...
221