Biological assessment of contaminated shooting range soil using earthworm biomarkers

Environmental contamination at shooting ranges is a widely known ecological problem. The aim of the study was to evaluate the extent of contamination and the ecotoxicity of a shooting range soil assessing the physiological and biochemical effects on earthworm Eisenia fetida (Savigny). Adult E. fetida were exposed to the soils collected from different distances of the shooting range for 28 days. High concentrations of Pb (53023 mg kg^-1), increased concentrations of Ni (12 mg kg^-1) and Sb (600 mg kg^-1), significantly higher soil organic matter content (7.2%) and density (6.14 g cm^-3) were determined in the backstop berm soil. Significant weight loss (44.4%) of the adult earthworms after 28 days of exposure occurred in the most contaminated shooting range soil and significantly higher concentrations of Pb (3101 mg kg^-1), Cu (51 mg kg^-1), Ni (2 mg kg^-1), and Sb (20 mg kg^-1) were determined in the tissues of worms, and no juveniles found there. Juveniles exposed to the less contaminated soil of the shooting range (A, B and C) accumulated significant concentrations of Pb, Cu, Fe, Mn, and Zn. The antioxidant enzymatic activity (glutathione-s-transferase (GST)) decreased, and lipid peroxidation increased as indicated by an increase in malondialdehyde (MDA) level in earthworms exposed to the contaminated soil. A compensatory mechanism between the activities of glutathione reductase (GR) and GST in earthworms exposed to these soils was confirmed.