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Related Experiment Video

Updated: Oct 19, 2025

Detection of SARS-CoV-2 Neutralizing Antibodies using High-Throughput Fluorescent Imaging of Pseudovirus Infection

Detection of SARS-CoV-2 Neutralizing Antibodies using High-Throughput Fluorescent Imaging of Pseudovirus Infection

Published on: June 5, 2021

A SARS-CoV-2 Neutralization Assay Using Single Molecule Arrays.

Tal Gilboa^1,2,3, Limor Cohen^1,2,3, Chi-An Cheng^1,2,3

¹Harvard Medical School, Boston, MA, 02115, USA.

Angewandte Chemie (International Ed. in English)

|September 17, 2021

Summary

This summary is machine-generated.

A new assay using Single Molecule Arrays (Simoa) measures COVID-19 antibody neutralization quickly and sensitively. This tool aids in analyzing immune responses in infected and vaccinated individuals, supporting vaccine development and studies.

Keywords:

COVID-19 multiplexing neutralization assays single molecule arrays

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In Vitro Selection of Aptamers to Differentiate Infectious from Non-Infectious Viruses

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Last Updated: Oct 19, 2025

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In Vitro Selection of Aptamers to Differentiate Infectious from Non-Infectious Viruses

In Vitro Selection of Aptamers to Differentiate Infectious from Non-Infectious Viruses

Published on: September 7, 2022

Area of Science:

Immunology
Virology
Biotechnology

Background:

Coronavirus disease 2019 (COVID-19) presents diverse clinical symptoms, necessitating precise assays for immune response evaluation.
Existing methods for analyzing antibody neutralization can be complex and time-consuming.

Purpose of the Study:

To develop a sensitive, rapid, and adaptable assay for measuring antibody neutralization of SARS-CoV-2.
To evaluate the assay's performance in diverse patient cohorts and against different viral variants.

Main Methods:

Development of a Single Molecule Array (Simoa) based assay measuring antibody-mediated blockage of the ACE2-spike interaction.
Assay validation using samples from patients with varying COVID-19 severity and from vaccinated individuals.
Adaptation of the assay for SARS-CoV-2 variants.

Main Results:

The Simoa assay demonstrated high sensitivity and multiplexing capabilities for assessing antibody neutralization.
The assay successfully measured increasing neutralization and antibody levels over time in COVID-19 patients.
Effective measurement of neutralization capacity against SARS-CoV-2 variants in different populations was achieved.

Conclusions:

The developed Simoa assay offers a rapid, sensitive, and versatile tool for evaluating COVID-19 immunity.
This assay is suitable for clinical applications, including vaccine efficacy studies and epidemiological research.
The assay's adaptability supports ongoing monitoring of viral evolution and immune responses.