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Measuring the effects of postmortem time and age on mouse lens elasticity using atomic force microscopy
Wyndham More Batchelor1, Bianca Maceo Heilman2, Esdras Arrieta-Quintero3
1Biomedical Atomic Force Microscopy Laboratory, Department of Biomedical Engineering, University of Miami College of Engineering, Miami, FL, USA.
Experimental Eye Research
|September 17, 2021
Summary
Mouse lenses, crucial for ophthalmic research, show age-related stiffness. However, postmortem time significantly impacts biomechanical analysis, highlighting the need for controlled experimental conditions.
Area of Science:
- Ophthalmology
- Biophysics
- Materials Science
Background:
- The mouse lens is a common model for human lens conditions like presbyopia.
- Mouse lenses are susceptible to structural and biomechanical changes post-extraction.
- These changes can interfere with accurate biomechanical analysis.
Purpose of the Study:
- To investigate the impact of aging and postmortem time on mouse lens biomechanics.
- To quantify changes in Young's Modulus of Elasticity in mouse lenses.
- To emphasize the importance of controlling experimental variables in lens research.
Main Methods:
- Atomic force microscopy (AFM) microindentation was employed.
- Young's Modulus of Elasticity was measured.
- Mouse lenses were analyzed at different ages and postmortem intervals.
Main Results:
- Older mouse lenses were stiffer than younger ones immediately postmortem (p=0.028).
- After 18 hours of incubation, no significant difference in stiffness was observed between age groups (p=0.997).
- Postmortem time significantly influences measured lens stiffness.
Conclusions:
- Experimental conditions, particularly postmortem time, must be strictly controlled.
- Mouse lens biomechanical properties are sensitive to handling and storage.
- Standardized protocols are essential for reliable ophthalmic research using mouse models.
