Identification of SRSF3 target mRNAs using inducible TRIBE

Area of Science:

• Molecular Biology
• Genetics
• Stem Cell Biology

Background:

• Serine and arginine-rich splicing factor 3 (SRSF3) is a key regulator of gene expression.
• Its precise RNA targets and recognition mechanisms in human pluripotent stem cells remain largely unknown.
• SRSF3 plays critical roles in embryonic development, reprogramming, and maintaining pluripotency.

Purpose of the Study:

• To identify the direct RNA targets of SRSF3 in human embryonic stem cells (hESCs).
• To elucidate the RNA recognition specificity of SRSF3.
• To provide a comprehensive resource for understanding SRSF3 function in hESCs.

Main Methods:

• Utilized inducible TRIBE (targets of RNA binding sites by editing) technology.
• Applied motif analysis to SRSF3 binding sites.
• Integrated data to map SRSF3 binding sites and gene targets.

Main Results:

• Identified 3888 confident SRSF3 binding sites, targeting 1222 genes.
• Nearly half of the binding sites were located in exons, supporting SRSF3's role in alternative splicing.
• Discovered conserved SRSF3 recognition sequences between human and mouse embryonic stem cells.

Conclusions:

• This study reveals the comprehensive RNA targets of SRSF3 in hESCs.
• Uncovered the specific RNA recognition motifs utilized by SRSF3.
• Provides essential data for future research on SRSF3's function in pluripotency and development.