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Published on: May 30, 2018
Raman spectroscopy of healthy, injured and amniotic membrane treated rat spinal cords
Elisabeth Salmagi Teixeira Coutinho1, Lázaro Pinto Medeiros Neto2, Tanmoy Bhattacharjee3
1Laboratory of Histology and Regenerative Therapy, Institute for Research and Development (IP&D), Universidade do Vale do Paraíba (UniVap), Av. Shishima Hifumi, 2911, Urbanova, São José dos Campos, 12244-000 São Paulo (SP), Brazil.
Abstract:
Spinal cord injury is a significant public health issue with high psychological and financial costs to both the family and the society. Effective treatment strategies are hence of immense value. Several reports have suggested application of amniotic membrane for treating injuries, and there is evidence that it may be used to treat spinal injuries. In this animal model study, we explore biochemical changes in amniotic membrane treated injured spinal cord with respect to untreated injured and uninjured spinal cord using Raman spectroscopy. Multivariate statistical analysis is able to classify control, untreated, and treated with 92%, 87%, and 80% efficiency, respectively; suggesting unique biochemical changes in each group. Such studies may lead to development of minimally invasive methodologies for spinal cord injury treatment monitoring.
