Related Experiment Video
Updated: Oct 19, 2025

A Novel Bioreactor for High Density Cultivation of Diverse Microbial Communities
Published on: December 25, 2015
Enhanced nitrogen removal by partial nitrification-anammox process with a novel high-frequency micro-aeration (HFMA)
Yulei Chi1, Xuan Shi1, Pengkang Jin1
1School of Human Settlements and Civil Engineering, Xi'an Jiaotong University, Xi'an, Shaanxi Province 710049, China; School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an, Shaanxi Province 710055, China.
Abstract:
A novel high-frequency micro-aeration (HFMA) mode with aeration frequency of 15 times/h and DO concentration lower than 0.5 mg/L was proposed. Advanced partial nitrification-anammox (PN-A) performance was achieved in a two-stage sequencing batch reactor-integrated fixed-film activated sludge reactor with the HFMA mode. When treating wastewater with carbon/nitrogen ratio of 3, the abundance of NO2--N oxidation related genes decreased, and the genes carried out NO3--N reduction and carbon source consumption were up-regulated. These variations in microbial metabolism brought more NO2--N substrate for the subsequent anammox process, and consumed part of the accumulated organic matter and NO3--N. Thus, the HFMA conditions eventually promoted the expression of anammox bacteria with NH2OH as an intermediate metabolite and the substance exchange activity of anammox bacteria. The changes in microorganisms lead to increase in the nitrite accumulation rate, nitrogen removal efficiency and abundance of anammox bacteria (16.34%, 18.71% and 5.92%, respectively).
More Related Videos
07:34Author Spotlight: Scaling Microalgal Biotechnology for Enhanced Biomethane Production
Published on: March 22, 2024
08:05Measurement of the Potential Rates of Dissimilatory Nitrate Reduction to Ammonium Based on 14NH4+/15NH4+ Analyses via Sequential Conversion to N2O
Published on: October 7, 2020
Related Concept Videos
Metabolism of Chemolithotrophs
Inorganic Nitrogen Assimilation
Environmental Applications of Microorganisms
Overview of Nitrogen Metabolism
The largest pool of nitrogen available in the terrestrial ecosystem is gaseous nitrogen (N2) from the air, but this...
Bioremediation
Microbial Nutrition