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Class I myosin mediated endocytosis and polarization growth is essential for pathogenicity of Magnaporthe oryzae

Chengcheng Zheng1,2, Weiwei Zhang1,2, Shulin Zhang1,2

  • 1Key Laboratory of Biology and Sustainable Management of Plant Diseases and Pests of Anhui Higher Education Institutes, Anhui Agricultural University, 130 West of Changjiang Road, Hefei, 230036, China.

Applied Microbiology and Biotechnology
|September 18, 2021
PubMed

Insights

The class I myosin protein, Momyo1, is crucial for the growth, pigmentation, and virulence of Magnaporthe oryzae. Knockdown mutants show defects in cell wall integrity, endocytosis, and appressorium formation, highlighting Momyo1's essential roles.

Area of Science:

  • Mycology
  • Molecular Biology
  • Cell Biology

Background:

  • Myosin proteins are essential for numerous cellular processes in eukaryotes.
  • Class II and V myosins are known to be important in Magnaporthe oryzae for growth and pathogenicity.
  • The function of class I myosin in M. oryzae remained largely uncharacterized.

Purpose of the Study:

  • To investigate the biological function of the class I myosin protein, Momyo1, in the rice blast fungus Magnaporthe oryzae.
  • To elucidate Momyo1's role in fungal development, cell wall integrity, endocytosis, and pathogenicity.

Main Methods:

  • Gene expression analysis showing high Momyo1 expression during conidiation and infection.
  • Functional characterization using RNA interference (RNAi) to create Momyo1 knockdown mutants.
  • Analysis of mutant phenotypes including growth, conidiation, pigmentation, appressorium formation, cell wall integrity, hydrophobicity, endocytosis, and Spitzenkörper formation.
  • Protein-protein interaction studies to identify interacting partners, such as MoShe4.

Main Results:

  • Momyo1 is highly expressed during conidiation and infection stages.
  • Momyo1 is required for vegetative growth, conidiation, melanin pigmentation, and pathogenicity.
  • Momyo1 knockdown mutants exhibit defects in appressorium-like structure formation, cell wall integrity, hyphal hydrophobicity, endocytosis, and Spitzenkörper formation.
  • Momyo1 physically interacts with MoShe4, a protein potentially involved in endocytosis and actin cytoskeleton polarization.

Conclusions:

  • Momyo1 plays a critical role in regulating vegetative growth, pigmentation, cell wall integrity, endocytosis, and virulence in Magnaporthe oryzae.
  • Momyo1 is essential for proper hyphal development, including appressorium formation, and surface properties.
  • The interaction of Momyo1 with MoShe4 suggests a coordinated mechanism for endocytosis and cytoskeletal organization impacting fungal pathogenicity.
Keywords:
Cell wall integrityEndocytosisMagnaporthe oryzaeMomyo1Pathogenicity

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