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Updated: Oct 19, 2025

Large-scale Three-dimensional Imaging of Cellular Organization in the Mouse Neocortex

Large-scale Three-dimensional Imaging of Cellular Organization in the Mouse Neocortex

Published on: September 5, 2018

Exploring the human cerebral cortex using confocal microscopy.

Luca Pesce¹, Annunziatina Laurino¹, Marina Scardigli¹

¹European Laboratory for Non-linear Spectroscopy (LENS), University of Florence, Sesto Fiorentino, FI, Italy; Department of Physics and Astronomy, University of Florence, Sesto Fiorentino, FI, Italy.

Progress in Biophysics and Molecular Biology

|September 18, 2021

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This summary is machine-generated.

Mapping human brain neurons is crucial for understanding brain function. This study introduces an efficient method for cellular mapping of the human cerebral cortex using advanced tissue clearing and immunostaining techniques.

Area of Science:

Neuroscience
Cellular Biology
Biotechnology

Background:

Understanding human brain structure at the cellular level is key to deciphering neural network functions.
Tissue clearing techniques have advanced, but challenges remain in labeling and clearing human brain samples.
Lipid-dissolving chemicals homogenize refractive indices for tissue transparency.

Purpose of the Study:

To develop and evaluate an efficient method for cellular mapping of the human cerebral cortex.
To couple immunostaining with SWITCH/TDE clearing and confocal microscopy for detailed brain mapping.
To assess autofluorescence and lipofuscin pigment interference during the process.

Main Methods:

Immunostaining of human cerebral cortex slices.

Keywords:

2′2-thiodiethanol Clearing Human brain Immunolabeling Lipofuscin SWITCH

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Last Updated: Oct 19, 2025

Large-scale Three-dimensional Imaging of Cellular Organization in the Mouse Neocortex

Large-scale Three-dimensional Imaging of Cellular Organization in the Mouse Neocortex

Published on: September 5, 2018

Visualization of Cortical Modules in Flattened Mammalian Cortices

Visualization of Cortical Modules in Flattened Mammalian Cortices

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In vivo Imaging of Deep Cortical Layers using a Microprism

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Application of SWITCH/TDE tissue clearing technique.

Confocal microscopy for high-resolution imaging.

Evaluation of autofluorescence and lipofuscin interference.

Main Results:

Demonstrated an efficient clearing and labeling process for adult human brain slices.
Successfully performed cellular mapping of the human cerebral cortex.
Provided a specific evaluation of autofluorescence and lipofuscin pigment interference.
Validated the method for morphological classification and antibody validation.

Conclusions:

The developed approach enables efficient cellular mapping of the human cerebral cortex.
This method is suitable for morphological classification and antibody validation of neuronal and non-neuronal markers.
Advances in tissue clearing and immunostaining offer new possibilities for brain research.