The pentatricopeptide repeat protein GEND1 is required for root development and high temperature tolerance in
Zhengfei Guo1, Xiaoyu Wang2, Zhubing Hu2
1College of Life Sciences, Nanjing Agricultural University, Nanjing, 210095, People's Republic of China; Key Laboratory of Plant Stress Biology, School of Life Sciences, Henan University, Kaifeng, 475004, People's Republic of China.
Abstract:
Pentatricopeptide repeat (PPR) proteins are a large family in land plants that play a role in organellular RNA processing, editing, and splicing. Here, we identify an Arabidopsis thaliana mutant, gend1-1, which exhibits a short root phenotype with reduced meristem size and cell numbers. Positional cloning of GEND1 revealed that it encodes a PPR protein, and functional analysis showed that GEND1 can bind and edit mitochondrial ccmFn-1 mRNA, causing gend1 mutants to have decreased levels of cytochrome C. GEND1 was up-regulated by high temperature conditions, to which gend1 mutants were hypersensitive. Analysis of a set of PPR mutants under high temperature showed that mutants with defects in cytochrome C had comparable temperature sensitivity to gend1. Collectively, these results suggest that cytochrome C plays an important role in root development and high temperature response in Arabidopsis.
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