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Updated: Oct 19, 2025

Fish Sperm Assessment Using Software and Cooling Devices

Fish Sperm Assessment Using Software and Cooling Devices

Published on: July 28, 2018

Assessing different liquid-storage temperatures for rooster spermatozoa.

Marcel H Blank¹, Lourenço P Ruivo¹, Gabriel A Novaes¹

¹Group of Study for Avian Multiplication, Department of Animal Reproduction, School of Veterinary Science, University of São Paulo, São Paulo, Brazil.

Animal Reproduction Science

|September 19, 2021

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Storing chicken semen at 5°C for extended periods shows minimal impact on sperm function. However, higher temperatures (25°C and 37°C) significantly damage sperm motility, membrane integrity, and chromatin, especially with longer storage times.

Area of Science:

Animal Science
Reproductive Biology
Sperm Cryopreservation

Background:

Liquid storage of poultry semen is common, but optimal temperature and time parameters remain unclear.
Understanding the effects of storage conditions on sperm physiology is crucial for artificial insemination success.

Purpose of the Study:

To investigate the impact of different storage temperatures (5°C, 25°C, 37°C) and durations on various chicken semen variables.
To determine the threshold for sperm damage under different liquid storage conditions.

Main Methods:

Semen samples were stored at 5°C, 25°C, and 37°C for varying durations.
Evaluated sperm motility, mitochondrial function, acrosomal integrity, plasma membrane integrity (using eosin-negrosin and propidium iodide staining), chromatin dispersion, chromatin integrity, and oxidative stress markers.

Keywords:

Cooling Poultry Preservation Semen Sperm quality

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Last Updated: Oct 19, 2025

Fish Sperm Assessment Using Software and Cooling Devices

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Published on: July 28, 2018

Sperm Collection of Differential Quality Using Density Gradient Centrifugation

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Published on: November 29, 2018

Vitrification of In Vitro Matured Oocytes Collected from Adult and Prepubertal Ovaries in Sheep

Vitrification of In Vitro Matured Oocytes Collected from Adult and Prepubertal Ovaries in Sheep

Published on: July 10, 2021

Main Results:

Storage at 37°C was most detrimental, causing damage within 4 hours.
Progressive motility and mitochondrial function declined within 2 hours at 25°C and 37°C, and 4 hours at 5°C.
Acrosomal and plasma membrane damage occurred at 37°C; chromatin integrity was compromised after 4 hours at 5°C and 25°C, and 2 hours at 37°C.

Conclusions:

Prolonged storage at 5°C results in minimal changes to chicken spermatozoa.
Short-term storage at 25°C did not appear to cause significant sperm damage.
Higher temperatures (37°C) rapidly impair multiple sperm functions, highlighting the importance of controlled storage conditions.