Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Nucleic acids02:43

Nucleic acids

Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and carry instructions for its functioning.
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes, the...
Next-generation Sequencing03:00

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features.
RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...
Nucleic Acids and Nucleotides01:20

Nucleic Acids and Nucleotides

Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and have instructions for its functioning. The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA).
Deoxyribonucleic Acid (DNA)
DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and the organelles such as chloroplasts and mitochondria. In...
Nucleic Acid Structure01:25

Nucleic Acid Structure

The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group on the ribose's second carbon and a hydrogen on the deoxyribose's second carbon. The phosphate residue attaches to the hydroxyl group of the 5′ carbon of one sugar and the hydroxyl group of the 3′ carbon of the sugar of the next nucleotide, which forms  a 5′ to 3′ phosphodiester linkage.
DNA Structure
DNA has a double-helix structure. The...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Editorial: Cell models and preclinical validation of immune-mediating biological therapies.

Frontiers in immunology·2026
Same author

Suicidality phenotypes reflect both shared and distinct genetic factors.

medRxiv : the preprint server for health sciences·2026
Same author

Epigenetic Evidence Implies Disturbed Proteostasis and Potentially Protein Aggregation in Suicidality.

Biomolecules·2026
Same author

Transposable elements as a possible missing link between genetic predisposition and environmental triggers of autoimmune disorders: insights from type 1 diabetes, systemic lupus erythematosus and rheumatoid arthritis.

Frontiers in immunology·2026
Same author

Metabolomic Profiling of Extracellular Vesicles Reveals Distinct Metabolic Dysregulation and Treatment-Specific Signatures in Depression.

Biomolecules·2026
Same author

Variants in <i>TPH2</i> and <i>ADARB1</i> genes in completed suicide from the Slovenian population - a follow-up to findings on attempted suicide in the Serbian population.

The world journal of biological psychiatry : the official journal of the World Federation of Societies of Biological Psychiatry·2026

Related Experiment Video

Updated: Jun 20, 2026

Protocol for the Solid-phase Synthesis of Oligomers of RNA Containing a 2'-O-thiophenylmethyl Modification and Characterization via Circular Dichroism
11:37

Protocol for the Solid-phase Synthesis of Oligomers of RNA Containing a 2'-O-thiophenylmethyl Modification and Characterization via Circular Dichroism

Published on: July 28, 2017

19.3K

OligoPrime: An Information System for Oligonucleotide Management.

Šimen Ravnik1, Ines Žabkar2, Uršula Prosenc Zmrzljak3

  • 1Faculty of Computer and Information Science, University of Ljubljana, Ljubljana, Slovenia.

Biomedical Engineering and Computational Biology
|September 20, 2021
PubMed
Summary

OligoPrime is a new web-based system designed to manage oligonucleotides throughout their lifecycle in research. This tool enhances traceability, sharing, and ordering of essential molecular biology reagents.

Keywords:
Oligonucleotidecomputational supportinformation systemmanagementprimerweb application

More Related Videos

Chemical Triphosphorylation of Oligonucleotides
13:19

Chemical Triphosphorylation of Oligonucleotides

Published on: June 2, 2022

3.7K
Author Spotlight: Characterizing Novel Enzymes from Extremophiles and Common Pathogens to Understand DNA Repair and Replication
05:33

Author Spotlight: Characterizing Novel Enzymes from Extremophiles and Common Pathogens to Understand DNA Repair and Replication

Published on: July 5, 2024

913

Related Experiment Videos

Last Updated: Jun 20, 2026

Protocol for the Solid-phase Synthesis of Oligomers of RNA Containing a 2'-O-thiophenylmethyl Modification and Characterization via Circular Dichroism
11:37

Protocol for the Solid-phase Synthesis of Oligomers of RNA Containing a 2'-O-thiophenylmethyl Modification and Characterization via Circular Dichroism

Published on: July 28, 2017

19.3K
Chemical Triphosphorylation of Oligonucleotides
13:19

Chemical Triphosphorylation of Oligonucleotides

Published on: June 2, 2022

3.7K
Author Spotlight: Characterizing Novel Enzymes from Extremophiles and Common Pathogens to Understand DNA Repair and Replication
05:33

Author Spotlight: Characterizing Novel Enzymes from Extremophiles and Common Pathogens to Understand DNA Repair and Replication

Published on: July 5, 2024

913

Area of Science:

  • Molecular Biology
  • Bioinformatics
  • Laboratory Management

Background:

  • Increasing use of molecular biology techniques generates large volumes of oligonucleotides.
  • Current management practices can lead to traceability and sharing challenges.
  • A dedicated system is needed to streamline oligonucleotide lifecycle management.

Purpose of the Study:

  • To introduce OligoPrime, a novel electronic oligonucleotide management system.
  • To provide computational support for oligonucleotide ordering, storage, application, and disposal.
  • To enhance oligonucleotide traceability and facilitate laboratory sharing and bulk ordering.

Main Methods:

  • OligoPrime is a web-accessible information system.
  • It requires no end-user installation.
  • Features include filtering and searching by location, sequence, and availability.
  • Database sharing with role-based access permissions.
  • Built on open-source software.

Main Results:

  • OligoPrime offers a centralized platform for oligonucleotide management.
  • The system simplifies searching, tracking, and sharing of oligonucleotides.
  • User roles ensure appropriate access control within research groups.
  • The system is easily managed and installed.

Conclusions:

  • OligoPrime is a unique software solution for dedicated oligonucleotide management.
  • It significantly improves transparency and simplifies oligonucleotide handling in academic labs.
  • The system supports the entire oligonucleotide lifecycle from procurement to disposal.