Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Eukaryotic RNA Polymerases00:58

Eukaryotic RNA Polymerases

25.1K
RNA Polymerase (RNAP) is conserved in all animals, with bacterial, archaeal, and eukaryotic RNAPs sharing significant sequence, structural, and functional similarities. Among the three eukaryotic RNAPs, RNA Polymerase II is most similar to bacterial RNAP in terms of both structural organization and folding topologies of the enzyme subunits. However, these similarities are not reflected in their mechanism of action.
All three eukaryotic RNAPs require specific transcription factors, of which the...
25.1K
Replication in Eukaryotes01:29

Replication in Eukaryotes

15.3K
In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...
15.3K
RNA Stability01:53

RNA Stability

34.2K
Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
34.2K
Telomeres and Telomerase02:41

Telomeres and Telomerase

24.9K
In eukaryotic DNA replication, a single-stranded DNA fragment remains at the end of a chromosome after the removal of the final primer. This section of DNA cannot be replicated in the same manner as the rest of the strand because there is no 3’ end to which the newly synthesized DNA can attach. This non-replicated fragment results in gradual loss of the chromosomal DNA during each cell duplication. Additionally, it can induce a DNA damage response by enzymes that recognize single-stranded...
24.9K
Bacterial RNA Polymerase00:43

Bacterial RNA Polymerase

30.9K
Unlike eukaryotes, bacteria use a single RNA Polymerase (RNAP) to transcribe all genes. The different subunits of bacterial RNAPhave distinct functions. The multisubunit structure of the bacterial RNAP helps the enzyme to maintain catalytic function, facilitate assembly, interact with DNA and RNA, and self-regulate its activity.
In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...
30.9K
Proofreading01:31

Proofreading

7.1K
Synthesis of new DNA molecules is carried out by the enzyme DNA polymerase, which adds nucleotides on the daughter strand complementary to the template DNA strand. DNA polymerase has a higher affinity to add the correct base and ensures fidelity during DNA replication. Furthermore,  it exhibits proofreading activity during replication, using an exonuclease domain that cuts off incorrect nucleotides from the nascent DNA strand.
Errors During Replication are Corrected by the DNA Polymerase...
7.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Remembrance of things past: Towards a life-course biology of aging.

PLoS biology·2026
Same author

ß-adrenergic-like signalling engages CrebB in Drosophila gut to promote female longevity.

Nature communications·2026
Same author

Upcycling of Waste Cherries Produces an Anthocyanin-Rich Powder That Protects Against Amyloid-β Toxicity in <i>C. elegans</i>.

Antioxidants (Basel, Switzerland)·2025
Same author

Loss of Pol III repressor Maf1 in neurons promotes longevity by preventing the age-related decline in 5S rRNA and translation.

PLoS biology·2025
Same author

Senescence Cell Induction Methods Display Diverse Metabolic Reprogramming and Reveal an Underpinning Serine/Taurine Reductive Metabolic Phenotype.

Aging cell·2025
Same author

Overlooked histories in ageing research: Pioneering women at the foundation of our field.

Aging cell·2024

Related Experiment Video

Updated: Oct 19, 2025

A Suppressor Screen for the Characterization of Genetic Links Regulating Chronological Lifespan in Saccharomyces cerevisiae
10:39

A Suppressor Screen for the Characterization of Genetic Links Regulating Chronological Lifespan in Saccharomyces cerevisiae

Published on: September 17, 2020

6.5K

Corrigendum: RNA Polymerase III, Ageing and Longevity.

Yavuz Kulaberoglu1, Yasir Malik2, Gillian Borland3

  • 1Department of Genetics Evolution and Environment, Institute of Healthy Ageing, University College London, London, United Kingdom.

Frontiers in Genetics
|September 20, 2021
PubMed
Summary

This study corrects the article DOI for a genetics research paper. The correction ensures proper citation and discoverability of the published genetic findings.

Keywords:
MAF1RNA polymerase IIITORC1ageingmTOR

More Related Videos

The Replica Set Method: A High-throughput Approach to Quantitatively Measure Caenorhabditis elegans Lifespan
11:58

The Replica Set Method: A High-throughput Approach to Quantitatively Measure Caenorhabditis elegans Lifespan

Published on: June 29, 2018

9.6K
Lipid Supplementation for Longevity and Gene Transcriptional Analysis in Caenorhabditis elegans
07:25

Lipid Supplementation for Longevity and Gene Transcriptional Analysis in Caenorhabditis elegans

Published on: December 9, 2022

1.7K

Related Experiment Videos

Last Updated: Oct 19, 2025

A Suppressor Screen for the Characterization of Genetic Links Regulating Chronological Lifespan in Saccharomyces cerevisiae
10:39

A Suppressor Screen for the Characterization of Genetic Links Regulating Chronological Lifespan in Saccharomyces cerevisiae

Published on: September 17, 2020

6.5K
The Replica Set Method: A High-throughput Approach to Quantitatively Measure Caenorhabditis elegans Lifespan
11:58

The Replica Set Method: A High-throughput Approach to Quantitatively Measure Caenorhabditis elegans Lifespan

Published on: June 29, 2018

9.6K
Lipid Supplementation for Longevity and Gene Transcriptional Analysis in Caenorhabditis elegans
07:25

Lipid Supplementation for Longevity and Gene Transcriptional Analysis in Caenorhabditis elegans

Published on: December 9, 2022

1.7K

Area of Science:

  • Genetics
  • Bioinformatics

Context:

  • Correction of a previously published article DOI.
  • Ensuring accurate citation for genetic research.

Purpose:

  • To provide the correct Digital Object Identifier (DOI) for the article.
  • Facilitating accurate referencing and retrieval of genetic data.

Summary:

  • The article DOI has been corrected to 10.3389/fgene.2021.705122.
  • This ensures the integrity of scientific records.

Impact:

  • Improved accessibility to the genetic research findings.
  • Enhanced discoverability of the study within the scientific community.