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vB-Ea-5: a lytic bacteriophage against multi-drug-resistant Enterobacter aerogenes

Fatemeh Habibinava1, Mohammad Reza Zolfaghari1, Mohsen Zargar1

  • 1Department of Microbiology, Faculty of Basic Sciences, Qom Branch, Islamic Azad University, Qom, Iran.

Iranian Journal of Microbiology
|September 20, 2021
PubMed
Abstract

Insights

Researchers isolated a lytic bacteriophage, vB-Ea-5, effective against multi-drug-resistant Enterobacter aerogenes. This bacteriophage shows promise for phage therapy in treating resistant bacterial infections.

Area of Science:

  • Microbiology
  • Virology
  • Genomics

Background:

  • Multi-drug-resistant Enterobacter aerogenes poses a significant threat due to limited antibiotic treatment options.
  • Bacteriophages offer a potential alternative for combating multi-drug-resistant bacterial infections.

Purpose of the Study:

  • To isolate and characterize a lytic bacteriophage specifically targeting multi-drug-resistant Enterobacter aerogenes.
  • To evaluate the potential of bacteriophages as a therapeutic agent against resistant E. aerogenes.

Main Methods:

  • Isolation of a lytic bacteriophage from hospital wastewater.
  • Morphological and genetic characterization, including transmission electron microscopy and whole-genome sequencing.
  • Analysis of bacteriophage replication parameters (latent period, burst size, burst time).

Main Results:

  • A Myoviridae family bacteriophage, vB-Ea-5, was isolated and characterized.
  • vB-Ea-5 possesses a 135,324 bp genome with 212 open reading frames, including a host lysis protein (Holin).
  • The bacteriophage genome lacks antibiotic resistance and toxin genes, with specific replication and structural protein coding.

Conclusions:

  • The isolated bacteriophage vB-Ea-5 demonstrates lytic activity against multi-drug-resistant E. aerogenes.
  • vB-Ea-5 is a promising candidate for phage therapy applications in treating infections caused by multi-drug-resistant E. aerogenes.
Keywords:
BacteriophagesDrug resistanceEnterobacter aerogenesMyoviridaeWaste water

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