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Updated: Oct 19, 2025
A Neonatal Imaging Model of Gram-Negative Bacterial Sepsis
Published on: August 12, 2020
Parechovirus and enteroviruses among young infants with sepsis in Iran
Manoochehr Makvandi1, Ali Teimoori1, Roya Pirmoradi1
1Infectious and Tropical Diseases Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Insights
Human parechoviruses (HPeV) and Human enteroviruses (EV) are common causes of sepsis in infants. This study found HPeV genotype 1 in 5% and EV in 38% of infants with sepsis in Iran, indicating their prevalence in the region.
Area of Science:
- Virology
- Pediatric Infectious Diseases
- Public Health
Background:
- Human parechoviruses (HPeV) and Human enteroviruses (EV) are significant pathogens causing sepsis-like illnesses in infants under three months old.
- Early diagnosis and identification of causative agents are crucial for managing sepsis in neonates and young infants.
Purpose of the Study:
- To determine the prevalence of HPeV and EV in infants younger than three months presenting with clinical signs of sepsis in Ahvaz, Iran.
- To identify the specific genotype of HPeV detected in the study population.
Main Methods:
- Blood samples were collected from 100 infants (under 90 days) with sepsis-like symptoms.
- RNA was extracted and tested for HPeV (VP1 region) and EV (5' UTR) using RT-PCR.
- HPeV positive samples underwent sequencing for genotyping and phylogenetic analysis.
Main Results:
- Human parechovirus (HPeV) was detected in 5% of infants (3% males, 2% females).
- All detected HPeV strains were identified as genotype 1.
- Human enterovirus (EV) was detected in 38% of infants (22% males, 16% females).
Conclusions:
- HPeV genotype 1 and EV are prevalent causes of sepsis among young infants in Ahvaz, Iran.
- The findings highlight the importance of HPeV and EV surveillance in infant sepsis cases within this region.
Background And Objectives:
Human parechoviruses (HPeV) and Human enteroviruses (EV) frequently cause a sepsis-like illness in young infants (younger than three months). Therefore, this study was conducted to determine the frequency of HPeV and EV among the young infants with clinical signs and symptoms of sepsis in Ahvaz city, Iran.
Materials And Methods:
The blood specimens were collected from 100 (younger than 90 days hospitalized infants) including 54 (56.25%) males and 46 (43.75%) females with clinical signs and symptoms of sepsis-like disease. The RNA was extracted and tested for detection of VP1 region of HPeV and 5 UTR (Untranslated Region) of EV by RT-PCR. The sequences of positive of HPeV were further analyzed to determine HPeV genotyping.
Results:
5/100 (5%) of patients including 2/46 (2%) females and 3/54 (3%) males tested positive for HPeV (P=0.85). The analysis of 5 positive VP1 region of HPeV revealed the genotype 1. The analysis of sequencing and phylogenetic tree revealed that the isolated HPeVs were genotype 1. While 38/100 (38%) specimens including 16 (16%) females and 22 (22%) males were tested positive for EV (P=0.68).
Conclusion:
The frequency of HPeV genotype 1 was 5% among the young infants with sepsis. While frequency of EV was 38% among the young infants with sepsis. This study showed HPeV genotype 1 and EV are dominant in this region.
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