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Related Experiment Video

Updated: Oct 19, 2025

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Applying TADF Emitters in Bioimaging and Sensing-A Novel Approach Using Liposomes for Encapsulation and Cellular

Poppy O Smith1, Dominic J Black1, Robert Pal1

  • 1Department of Chemistry, Durham University, Durham, United Kingdom.

Frontiers in Chemistry
|September 20, 2021
PubMed
Summary

Researchers developed a novel liposome delivery method for thermally activated delayed fluorescence (TADF) complexes, enabling cellular uptake and cytoplasmic localization. This technique allows for potential TADF-based sensing applications, such as monitoring molecular oxygen.

Keywords:
bioimagingfluorescence microscopyliposomessensingthermally activated delayed fluorescence (TADF)

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Area of Science:

  • Biochemistry
  • Nanotechnology
  • Cell Biology

Background:

  • Thermally activated delayed fluorescence (TADF) complexes offer unique photophysical properties for various applications.
  • Efficient delivery and intracellular localization of TADF complexes remain a challenge for biological applications.
  • Liposomes are versatile nanocarriers for drug and molecule delivery.

Purpose of the Study:

  • To develop a method for the efficient delivery, cellular uptake, and intracellular localization of TADF complexes.
  • To demonstrate the feasibility of using liposomes as a vehicle for TADF complex delivery.
  • To investigate the potential of this method for enabling TADF-based sensing applications.

Main Methods:

  • Confinement of TADF complexes within liposomes.
  • Utilizing liposomes as delivery vehicles for cellular uptake in HepG2 cells.
  • Employing confocal and time-resolved fluorescence microscopy for intracellular localization and fluorescence analysis.
  • Developing procedures for oxygen removal during liposome preparation without structural disruption.

Main Results:

  • Successful confinement of TADF complexes in liposomes.
  • Demonstrated cellular uptake and cytoplasmic localization of liposomal TADF complexes in HepG2 cells.
  • Observed prompt and delayed fluorescence signals from internalized liposomal TADF.
  • Established a method for oxygen removal in liposome preparation.

Conclusions:

  • Liposomes can effectively deliver un-functionalized TADF complexes into cells.
  • The developed method facilitates intracellular localization of TADF complexes.
  • This approach paves the way for TADF-based biosensing, including molecular oxygen detection.