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Visualization of Candida albicans in the Murine Gastrointestinal Tract Using Fluorescent In Situ Hybridization
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Fluorescently Labelled Aerolysin (FLAER) Labelling of Candida albicans Cells
Sneh Lata Singh1, Sneha Sudha Komath1
1School of Life Sciences, Jawaharlal Nehru University, New Delhi, India.
Bio-Protocol
|September 20, 2021
Abstract:
In this protocol we describe a nonradiolabelled labelling of GPI anchor in Candida albicans. The method uses a fluorescent probe to bind specifically to GPI anchors so that the level of GPI-anchored proteins at the cell surface can be measured. The labelling does not need permeabilization of cells and can be carried out in vivo.
