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Functional Analysis of Connexin Channels in Cultured Cells by Neurobiotin Injection and Visualization
Philipp Wörsdörfer1, Klaus Willecke2
1Institute of Anatomy and Cell Biology, University of Würzburg, Koellikerstraße 6, 97070 Würzburg, Germany.
Bio-Protocol
|September 20, 2021
Abstract:
Functional gap junction channels between neighboring cells can be assessed by microinjection of low molecular weight tracer substances into cultured cells. The extent of direct intercellular communication can be precisely quantified by this method. This protocol describes the iontophoretic injection and visualisation of Neurobiotin into cultured cells.
