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Updated: Oct 19, 2025

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The Soft Agar Colony Formation Assay
Published on: October 27, 2014
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Soft Agar Colony Formation Assay as a Hallmark of Carcinogenesis
Feng Du1, Xiaodi Zhao1, Daiming Fan1
1State key Laboratory of Cancer Biology, National Clinical Research Center for Digestive Diseases and Xijing Hospital of Digestive Diseases, Fourth Military Medical University, Xi'an, China.
Bio-Protocol
|September 20, 2021
Abstract:
Soft agar colony formation assay is established to estimate the anchorage-independent growth ability of cells. In this assay, a bottom layer of agar with complete media is poured and solidified first, followed by an upper layer containing a specified number of cells suspended in medium-agar mixture. After two weeks of incubation, the number of colonies will be counted, serving as an indicator of malignancy of tumor cells.

