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Assaying Glycogen and Trehalose in Yeast
1Department of Molecular Genetics and Microbiology, Stony Brook University, Stony Brook, NY, USA.
Bio-Protocol
|September 20, 2021
Summary
This study presents simple assays to measure stored carbohydrates, glycogen and trehalose, in yeast. These methods can reveal the yeast
Area of Science:
- Biochemistry
- Molecular Biology
- Yeast Biology
Background:
- Organisms store carbohydrates for energy.
- Yeast store carbohydrates as glycogen and trehalose.
- Carbohydrate levels reflect physiological state.
Purpose of the Study:
- To develop easy and streamlined assays for yeast glycogen and trehalose.
- To enable the assessment of yeast physiological state through carbohydrate levels.
- To provide methods applicable to both low and moderate-throughput analyses.
Main Methods:
- Development of simplified biochemical assays.
- Quantification of glycogen and trehalose in yeast samples.
- Adaptation of assays for varying sample throughput.
Main Results:
- Established straightforward assays for yeast glycogen and trehalose.
- Demonstrated the utility of these assays for determining physiological state.
- Validated methods for both small-scale and moderately high-throughput applications.
Conclusions:
- The developed assays provide accessible tools for measuring yeast carbohydrate stores.
- Assessing glycogen and trehalose levels offers insights into yeast physiology.
- These streamlined methods facilitate research in yeast metabolism and physiology.
