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Updated: Oct 19, 2025

Assessment of Dictyostelium discoideum Response to Acute Mechanical Stimulation
Published on: November 9, 2017
Bacterial Survival in Dictyostelium
Regin Rønn1,2, Xiuli Hao2, Freja Lüthje1
1Department of Biology, University of Copenhagen, Copenhagen, Denmark.
Abstract:
We performed an assay to test the ability of different E. coli strains to survive inside amoebal cells after ingestion. In the assay we incubated bacteria together with cells of Dictyostelium discoideum for six hours. After co-incubation most of the uningested bacteria were removed by centrifugation and the remaining uningested bacteria were killed by gentamicin. Gentamicin is used because it does not penetrate into eukaryotic cells allowing the ingested bacteria to survive the antibiotic treatment, whereas bacteria outside the amoebal cells are killed.
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