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Related Experiment Video

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Vaginal HSV-2 Infection and Tissue Analysis.

Marie Beck Iversen1, Søren Riis Paludan1, Christian Kanstrup Holm1

  • 1Department of Biomedicine, Faculty of Health Sciences, Aarhus University, Aarhus, Denmark.

Bio-Protocol
|September 20, 2021
PubMed
Summary

This study details a vaginal mouse model for studying herpes simplex virus type 2 (HSV-2) mucosal immunity. The model effectively replicates HSV-2 infection and allows for immune response analysis.

Keywords:
ImmunologyMucosal immunologyVaginal infectionVirus infectionin vivo model

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Area of Science:

  • Immunology
  • Virology
  • Infectious Diseases

Background:

  • The vaginal murine model is crucial for studying mucosal immunity against HSV-2.
  • Depo-Provera treatment alters vaginal histology, mimicking the human endocervical epithelium, a key site for HSV-2 infection.
  • This model facilitates research into both innate and adaptive immune responses to HSV-2.

Purpose of the Study:

  • To present a detailed protocol for a vaginal murine HSV-2 infection model.
  • To investigate the utility of this model for studying mucosal immunity and viral spread.

Main Methods:

  • Mice were pre-treated with Depo-Provera followed by intra-vaginal inoculation with HSV-2.
  • Viral replication was assessed in the mucosal epithelium and central nervous system (CNS).
  • Immune responses were measured via cytokine detection (ELISA, qPCR) and leukocyte recruitment (flow cytometry).

Main Results:

  • HSV-2 replicated in the vaginal mucosal epithelium and spread to the CNS (spinal cord, brain stem, cerebrum, cerebellum).
  • Cytokine responses and leukocyte infiltration in the vagina were quantifiable.
  • The model demonstrated suitability for studying innate and adaptive immunity to HSV-2.

Conclusions:

  • The Depo-Provera-treated vaginal mouse model is a valuable tool for HSV-2 mucosal immunity research.
  • This model allows for comprehensive analysis of viral pathogenesis and host immune responses.
  • It supports investigations into therapeutic strategies and vaccine development for HSV-2.