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Related Concept Videos

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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CRISPR01:59

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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Related Experiment Video

Updated: Oct 19, 2025

Construction of CRISPR Plasmids and Detection of Knockout Efficiency in Mammalian Cells through a Dual Luciferase Reporter System
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Multiplex Gene Editing via CRISPR/Cas9 System in Sheep.

Yiyuan Niu1, Yi Ding1, Xiaolong Wang1

  • 1College of Animal Science and Technology, Northwest A&F University, Yangling, China.

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|September 20, 2021
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Summary

Scientists used CRISPR/Cas9 gene editing to modify multiple genes in sheep embryos. This powerful technology enables livestock improvement by targeting economically important traits simultaneously.

Keywords:
ASIPBCO2Cas9MSTNSheep

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Area of Science:

  • Animal Science
  • Biotechnology
  • Genetics

Background:

  • Sheep are essential large animal models in biomedical research for development and disease studies.
  • CRISPR/Cas9 gene editing technology offers precise genetic modification capabilities.

Purpose of the Study:

  • To provide a detailed protocol for generating genetically modified sheep using CRISPR/Cas9.
  • To demonstrate the simultaneous modification of multiple genes (MSTN, ASIP, BCO2) in sheep.

Main Methods:

  • Utilized CRISPR/Cas9 system for gene editing in one-cell-stage sheep embryos.
  • Co-injected Cas9 mRNA and guide RNAs targeting MSTN, ASIP, and BCO2 genes.
  • Developed and described methods for sgRNA design, plasmid construction, and detection in fibroblasts, embryos, and sheep.

Main Results:

  • Successfully modified multiple genes in sheep using the CRISPR/Cas9 system.
  • Established an efficient protocol for genetic modification of sheep embryos.
  • Demonstrated the feasibility of targeting multiple genes simultaneously for desired traits.

Conclusions:

  • The CRISPR/Cas9 system is a powerful tool for genetic modification in sheep.
  • This method can be applied for livestock improvement by targeting multiple genes controlling economically significant traits.
  • The detailed protocol facilitates reproducible genetic engineering in sheep models.