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Rice Lamina Joint Inclination Assay
Hsing-Yi Li1, Hsin-Mei Wang1, Seonghoe Jang1,2
1Biotechnology Center in Southern Taiwan (BCST) of Agricultural Biotechnology Research Center (ABRC), Academia Sinica, Tainan, Taiwan.
Abstract:
Brassinosteroids (BRs) promote rice lamina inclination. Recently, we showed that OsBUL1 knockout mutant rice (osbul1) is defective in brassinosteroid signaling ( Jang et al., 2017 ). To show that lamina joint inclination of osbul1 is less-sensitive than WT to exogenous brassinolide (BL) treatment in the lamina joint inclination bioassays, we applied the protocol presented below. The protocol focuses on: (1) how to prepare rice samples for the assay, and (2) how to treat BL exogenously. Finally, we have added a result showing lamina inclination between WT and osbul1 in BL solutions of various concentrations.

