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Polyamine and Paraquat Transport Assays in Arabidopsis Seedling and Callus
Haoxi Chai1, Yun Shen1, Huazhong Shi1
1Department of Chemistry and Biochemistry, Texas Tech University, Lubbock, TX, USA.
Bio-Protocol
|September 20, 2021
Abstract:
Polyamines (PAs) are polycationic compounds found in all living organisms and play crucial roles in growth and survival. We here show the 'Polyamine and paraquat (PQ) transport assay' protocol, which can be used to examine the uptake activity of PA/PQ transporters. We have used this protocol to demonstrate that PUT3 in Arabidopsis is a polyamine transporter and is able to take up spermidine and its analog paraquat.
