Extraction of Soluble and Insoluble Protein Fractions from Mouse Brains and Spinal Cords
1Molecular Psychiatry, Dept. of Psychiatry and Psychotherapy, University Medical Center Göttingen (UMG), Göttingen, Germany.
Bio-Protocol
|September 20, 2021
Abstract:
The current protocol details the preparation of soluble and insoluble protein lysates from mouse brain or spinal cord samples. In detail, tissue homogenization and sequential protein extraction are described. This procedure yields soluble and insoluble protein extracts that can be further processed in down-stream applications like ELISA or Western blotting.
