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Updated: Oct 19, 2025
Subcellular Fractionation for the Isolation of Synaptic Components from the Murine Brain
Published on: September 14, 2022
Preparation of Crude Synaptosomal Fractions from Mouse Brains and Spinal Cords
1Molecular Psychiatry, Dept. of Psychiatry and Psychotherapy, University Medical Center Göttingen (UMG), Göttingen, Germany.
Abstract:
The current protocol describes the preparation of crude synaptosomal fractions from mouse brain or spinal cord samples. In detail, a sequential protocol yielding crude synaptosomal and light membrane fractions is provided. This fast and easy method might be sufficient to assess the amount of synaptic proteins in down-steam applications like Western-blot or ELISA in e.g., mouse models of Alzheimer's disease or other neurodegenerative conditions.
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