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Assessment of Modulation of Protein Stability Using Pulse-chase Method
1Department of Microbiology and Immunobiology, Max F. Perutz Laboratories, University of Vienna, Vienna, Austria.
Bio-Protocol
|September 20, 2021
Abstract:
Pulse-chase technique is a method widely used to assess protein or mRNA stability. The principle of pulse-chase relies on labeling proteins or mRNA produced during a short period of time called 'pulse' and then following the rate of disappearance of those labeled proteins over a period of time called 'chase'. This technique thus allows quantitative analysis of modulation of protein or mRNA stability under different treatments or culturing conditions.
