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Macrophage Survival Assay Using High Content Microscopy.

Remzi Onur Eren¹, Nicolas Fasel¹

¹Department of Biochemistry, University of Lausanne, Epalinges, Switzerland.

|September 20, 2021

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This study introduces a high-content microscopy method to precisely measure how different treatments affect macrophage survival. This approach offers a quantitative and unbiased way to study macrophage fate in tissue homeostasis.

Area of Science:

Immunology
Cell Biology
Tissue Homeostasis

Background:

Macrophages are crucial for maintaining tissue health by controlling inflammation and repair.
The survival and fate of macrophages directly influence tissue condition.
Understanding macrophage survival dynamics is key to understanding tissue homeostasis.

Purpose of the Study:

To develop and describe a high-content imaging protocol for quantifying macrophage survival.
To enable the assessment of various stimuli and pharmacological treatments on macrophage fate.
To provide a quantitative, unbiased, and high-throughput method for macrophage survival studies.

Main Methods:

Utilizing high-content microscopy for image acquisition.
Employing image processing software for quantitative analysis.

Keywords:

Cell survival High-content microscope Macrophage

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Designing a protocol to assess macrophage survival under diverse experimental conditions.

Main Results:

The protocol allows for quantitative and unbiased assessment of macrophage survival.
It enables high-throughput screening of different stimuli and drug combinations.
The method provides a robust measure of the impact on macrophage fate.

Conclusions:

This high-content imaging protocol is effective for studying macrophage survival.
It offers a valuable tool for research in immunology and tissue repair.
The method facilitates a deeper understanding of how treatments affect macrophage fate and tissue homeostasis.