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CRISPR/Cas9 Genome Editing

CRISPR/Cas9 Genome Editing

The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...

CRISPR and crRNAs

CRISPR and crRNAs

Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...

CRISPR

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...

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Related Experiment Video

Updated: Oct 19, 2025

A Rapid and Facile Pipeline for Generating Genomic Point Mutants in C. elegans Using CRISPR/Cas9 Ribonucleoproteins

A Rapid and Facile Pipeline for Generating Genomic Point Mutants in C. elegans Using CRISPR/Cas9 Ribonucleoproteins

Published on: April 30, 2018

Using CRISPR-ERA Webserver for sgRNA Design.

Honglei Liu¹, Xiaowo Wang², Lei S Qi^3,4,5

¹School of Biomedical Engineering, Capital Medical University, Beijing, China.

|September 20, 2021

Summary

This summary is machine-generated.

This protocol details using CRISPR-ERA, a webserver tool, for designing single-guide RNAs (sgRNAs) essential for CRISPR gene editing and regulation applications. It covers creating specific sgRNAs and genome-wide libraries for precise genetic modification.

Keywords:

CRISPR-Cas9 system Gene editing Gene regulation sgRNA design sgRNA library

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Genome Editing in Mammalian Cell Lines using CRISPR-Cas

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Last Updated: Oct 19, 2025

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Genome Editing in Mammalian Cell Lines using CRISPR-Cas

Genome Editing in Mammalian Cell Lines using CRISPR-Cas

Published on: April 11, 2019

Area of Science:

Molecular Biology
Genomics
Biotechnology

Background:

The CRISPR-Cas9 system offers powerful capabilities for both gene editing and gene regulation.
Effective application of CRISPR technology relies heavily on the accurate design of single-guide RNAs (sgRNAs).
Existing tools require optimization for comprehensive sgRNA design for diverse CRISPR applications.

Purpose of the Study:

To provide a protocol for designing sgRNA sequences using the CRISPR-ERA webserver.
To demonstrate the utility of CRISPR-ERA for both gene editing and gene regulation applications.
To guide users in creating sgRNA libraries for genome-wide studies.

Main Methods:

Utilizing the CRISPR-ERA webserver tool for sgRNA sequence design.
Implementing the protocol for designing specific sgRNAs targeting desired genes or genomic regions.
Applying the tool to generate genome-wide sgRNA libraries.

Main Results:

CRISPR-ERA facilitates the design of effective sgRNAs for CRISPR-Cas9 applications.
The webserver supports sgRNA design for both gene editing and gene regulation.
The protocol enables the creation of comprehensive, genome-wide sgRNA libraries.

Conclusions:

CRISPR-ERA is a valuable resource for researchers needing to design sgRNAs for CRISPR-based experiments.
The protocol simplifies the process of generating specific and genome-wide sgRNA libraries.
This tool enhances the accessibility and efficiency of CRISPR technology implementation.