Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Immunocytochemistry and Immunohistochemistry

Immunocytochemistry and Immunohistochemistry

Immunocytochemistry (ICC) and immunohistochemistry (IHC) are techniques that use antibodies to check for specific proteins or antigens in a sample. The technique was first published by Albert Coons in 1941 to detect the presence of pneumococcal antigen in tissue sections from mice infected with Pneumococcus. Immunocytochemistry helps localization of proteins or antigens in individual cells like blood cells, stem cells, etc., while immunohistochemistry does the same for tissue samples.
These...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

TaCNGC-2A suppresses seed dormancy and activates pre-harvest sprouting through modulating calcium and hormonal signaling pathways.

Nature communications·2026

Same author

Trehalose 6-phosphate as master switch for optimizing crop yield and resilience.

Trends in biotechnology·2026

Same author

The TaDof-2D-miR1832-TaP450-7A module regulates low-temperature-induced seed dormancy release in wheat.

Plant communications·2026

Same author

Synchronizing sucrose effluxers with influxers in phloem loading for yield output and adaptation to environment.

National science review·2025

Same author

Exogenous allantoin improves waterlogging tolerance of summer maize by regulating the endogenous allantoin and nitrogen metabolism.

Plant physiology and biochemistry : PPB·2025

Same author

Revisiting recent claims on the cellular expression, protein interaction, and functions of tomato cytosolic invertase2.

Plant physiology·2025

Search research articles

Related Experiment Video

Updated: Oct 19, 2025

Primer for Immunohistochemistry on Cryosectioned Rat Brain Tissue: Example Staining for Microglia and Neurons

Primer for Immunohistochemistry on Cryosectioned Rat Brain Tissue: Example Staining for Microglia and Neurons

Published on: May 12, 2015

Resin-embedded Thin-section Immunohistochemistry Coupled with Triple Cellular Counterstaining.

William M Palmer¹, John W Patrick¹, Yong-Ling Ruan¹

¹School of Environmental and Life Science, the University of Newcastle, Callaghan, Australia.

|September 20, 2021

Summary

This protocol uses combined staining techniques to visualize protein localization in plant vascular bundles. The method enhances cell structure visualization and is applicable to various plant tissues.

Keywords:

Immunolocalisation Microscopy Phloem Staining

More Related Videos

Combining Double Fluorescence In Situ Hybridization with Immunolabelling for Detection of the Expression of Three Genes in Mouse Brain Sections

Combining Double Fluorescence In Situ Hybridization with Immunolabelling for Detection of the Expression of Three Genes in Mouse Brain Sections

Published on: March 26, 2016

Visualization, Quantification, and Mapping of Immune Cell Populations in the Tumor Microenvironment

Visualization, Quantification, and Mapping of Immune Cell Populations in the Tumor Microenvironment

Published on: March 25, 2020

Related Experiment Videos

Last Updated: Oct 19, 2025

Primer for Immunohistochemistry on Cryosectioned Rat Brain Tissue: Example Staining for Microglia and Neurons

Primer for Immunohistochemistry on Cryosectioned Rat Brain Tissue: Example Staining for Microglia and Neurons

Published on: May 12, 2015

Combining Double Fluorescence In Situ Hybridization with Immunolabelling for Detection of the Expression of Three Genes in Mouse Brain Sections

Combining Double Fluorescence In Situ Hybridization with Immunolabelling for Detection of the Expression of Three Genes in Mouse Brain Sections

Published on: March 26, 2016

Visualization, Quantification, and Mapping of Immune Cell Populations in the Tumor Microenvironment

Visualization, Quantification, and Mapping of Immune Cell Populations in the Tumor Microenvironment

Published on: March 25, 2020

Area of Science:

Plant Biology
Cell Biology
Biochemistry

Background:

Vascular bundles in plants contain diverse cell types with unique properties.
Mature sieve elements are anucleated and feature callose-rich sieve plates.
Accurate protein localization is crucial for understanding plant physiology.

Purpose of the Study:

To develop a robust protocol for studying protein localization within plant vascular bundles.
To adapt the protocol for general application to resin-embedded plant tissues.
To combine immunohistochemistry with specific counterstaining methods for enhanced visualization.

Main Methods:

Utilized immunohistochemistry to detect cell wall invertase.
Employed aniline blue counterstaining to identify callose in sieve plates.
Applied DAPI for nucleus and calcofluor white for cell wall staining.
Ensured tissue section adherence using gelatin fixation for slide mounting.

Main Results:

Successfully visualized protein localization in conjunction with specific cell structures.
Demonstrated the presence of cell wall invertase within vascular bundle cells.
Validated the protocol's effectiveness through combined staining procedures.

Conclusions:

The developed protocol enables precise protein localization in plant vascular bundles.
This method is adaptable for diverse resin-embedded plant tissues.
Combined staining provides comprehensive cellular and subcellular localization data.