Gliding Assay to Analyze Microtubule-based Motor Protein Dynamics

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Albert Shim1, Gin Tezuka1, Luke Kupcha1

  • 1Department of Biology, University of Hawaii at Hilo, 200 West Kawili Street, Hilo, Hawaii 96720, USA.

Bio-Protocol
|September 20, 2021
PubMed

Abstract:

The purpose of this protocol is to provide an updated method of performing microtubule gliding assays and visualizing it using fluorescence microscopy.

Keywords:
Glass chamberGliding assayMitotic motorPolarity-marked microtubulesin vitro motility

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