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Updated: Oct 19, 2025
Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs
Published on: June 12, 2018
Biotinylated Micro-RNA Pull Down Assay for Identifying miRNA Targets
Pornima Phatak1,2, James M Donahue1,2
1Department of Surgery, University of Maryland School of Medicine, Baltimore, USA.
Abstract:
microRNA (miRNA) directly associates with its target transcripts (mRNA). This protocol describes a method for detection of direct interaction between miRNA and mRNA. The result of interaction helps screening the specific target mRNAs for a miRNA.
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