Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Patient-derived three-dimensional lung tumor models to evaluate response to therapy.

NPJ precision oncology·2026
Same author

ASO Visual Abstract: Surgery vs. Radiation for Stage 1A NSCLC in Nonagenarians: 20 Years of Data, Decisions, and Outcomes.

Annals of surgical oncology·2026
Same author

Surgery Versus Radiation for Stage 1A NSCLC in Nonagenarians: 20 Years of Data, Decisions, and Outcomes.

Annals of surgical oncology·2026
Same author

Esophageal Perforation Following Explosive Injury: A Case Report.

Journal of special operations medicine : a peer reviewed journal for SOF medical professionals·2025
Same author

Lung-resident SARS-CoV-2 peptide-specific immune responses in perfused 3D human lung explant models.

Frontiers in bioengineering and biotechnology·2025
Same author

Oral Diltiazem Prophylaxis for Atrial Fibrillation in Patients Undergoing Robotic Lobectomy.

Annals of thoracic surgery short reports·2025

Related Experiment Video

Updated: Oct 19, 2025

Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs
11:00

Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs

Published on: June 12, 2018

14.1K

Biotinylated Micro-RNA Pull Down Assay for Identifying miRNA Targets.

Pornima Phatak1,2, James M Donahue1,2

  • 1Department of Surgery, University of Maryland School of Medicine, Baltimore, USA.

Bio-Protocol
|September 20, 2021
PubMed
Summary

This study presents a new method to detect direct interactions between microRNAs (miRNAs) and messenger RNAs (mRNAs). This technique aids in identifying specific mRNA targets for a given miRNA.

Keywords:
Biotin labellingBiotin-pulldownTransfectionmiRNAmiRNA-mRNA interaction

More Related Videos

Detection of miRNA Targets in High-throughput Using the 3'LIFE Assay
12:49

Detection of miRNA Targets in High-throughput Using the 3'LIFE Assay

Published on: May 25, 2015

10.2K
Identifying Targets of Human microRNAs with the LightSwitch Luciferase Assay System using 3'UTR-reporter Constructs and a microRNA Mimic in Adherent Cells
07:19

Identifying Targets of Human microRNAs with the LightSwitch Luciferase Assay System using 3'UTR-reporter Constructs and a microRNA Mimic in Adherent Cells

Published on: September 28, 2011

36.4K

Related Experiment Videos

Last Updated: Oct 19, 2025

Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs
11:00

Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs

Published on: June 12, 2018

14.1K
Detection of miRNA Targets in High-throughput Using the 3'LIFE Assay
12:49

Detection of miRNA Targets in High-throughput Using the 3'LIFE Assay

Published on: May 25, 2015

10.2K
Identifying Targets of Human microRNAs with the LightSwitch Luciferase Assay System using 3'UTR-reporter Constructs and a microRNA Mimic in Adherent Cells
07:19

Identifying Targets of Human microRNAs with the LightSwitch Luciferase Assay System using 3'UTR-reporter Constructs and a microRNA Mimic in Adherent Cells

Published on: September 28, 2011

36.4K

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • microRNAs (miRNAs) are small non-coding RNAs that regulate gene expression post-transcriptionally.
  • miRNA function is mediated by direct binding to complementary sequences in target messenger RNAs (mRNAs).
  • Accurate identification of miRNA-mRNA interactions is crucial for understanding gene regulation.

Purpose of the Study:

  • To describe a novel protocol for the direct detection of miRNA-mRNA interactions.
  • To provide a method for screening specific mRNA targets of a particular miRNA.

Main Methods:

  • The protocol details a method for detecting direct physical association between individual miRNAs and their cognate mRNA transcripts.
  • This approach allows for the specific capture and identification of interacting miRNA-mRNA pairs.

Main Results:

  • The described method successfully detects direct interactions between miRNAs and their target mRNAs.
  • The results facilitate the screening and validation of specific mRNA targets for a given miRNA.

Conclusions:

  • The developed protocol offers a reliable way to confirm direct miRNA-mRNA binding.
  • This method is valuable for miRNA target validation and functional studies in molecular biology and genetics.