Mating Based Split-ubiquitin Assay for Detection of Protein Interactions
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Updated: Oct 19, 2025
Split-Ubiquitin Based Membrane Yeast Two-Hybrid MYTH System: A Powerful Tool For Identifying Protein-Protein Interactions
Published on: February 1, 2010
Wijitra Horaruang1, Ben Zhang1
1Laboratory of Plant Physiology and Biophysics, Bower Building, University of Glasgow, Glasgow, UK.
Abstract:
The mating based split-ubiquitin (mbSUS) assay is an alternative method to the classical yeast two-hybrid system with a number of advantages. The mbSUS assay relies on the ubiquitin-degradation pathway as a sensor for protein-protein interactions, and it is suitable for the determination of interactions between full-length proteins that are cytosolic or membrane-bound. Here we describe the mbSUS assay protocol which has been used for detecting the interaction between K+ channel and SNARE proteins ( Grefen et al., 2010 and 2015; Zhang et al., 2015 and 2016).
