The Current Challenges on Spray-Based Cell Delivery to the Skin Wounds

Area of Science:

• Regenerative Medicine and Tissue Engineering
• Dermatology and Wound Care

Background:

• Skin wounds, including burns and chronic ulcers, present significant clinical challenges.
• Conventional autologous skin grafting has limitations, including donor site morbidity.
• Cell spray technology offers a less invasive approach for skin regeneration.

Purpose of the Study:

• To review cell types and spray instruments used in cell delivery for skin regeneration.
• To discuss factors influencing cell viability and wound coverage in spray-based delivery.
• To highlight recent advances and clinical translation of cell spraying techniques.

Main Methods:

• Review of scientific literature on cell spray technology for wound treatment.
• Analysis of different cell sources (keratinocytes, fibroblasts, stem cells) and spray devices.
• Evaluation of physical and biological parameters affecting spray-based cell delivery efficiency.

Main Results:

• Cell spray decreases the required donor site area compared to traditional grafting.
• Cell viability and wound coverage are critical parameters influenced by spray settings and cell suspension properties.
• Advances include codelivery with matrices, growth factors, and biological glues.

Conclusions:

• Spray-based cell delivery is a promising therapeutic strategy for various skin wounds.
• Optimizing instruments and parameters is key to enhancing treatment efficacy.
• Further clinical translation of cell spraying holds potential for reducing morbidity and mortality associated with skin injuries.