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Related Experiment Video

Updated: Oct 19, 2025

Fabricating and Labeling Microbubbles with Fluorescent and Radioactive Tracers
10:40

Fabricating and Labeling Microbubbles with Fluorescent and Radioactive Tracers

Published on: January 24, 2025

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Preparation and Characterization of Targeted Microbubbles.

Galina B Diakova1, Matthew Wang1, Sunil Unnikrishnan1

  • 1Division of Cardiovascular Medicine, Department of Medicine and Robert M Berne Cardiovascular Research Center, Department of Biomedical Engineering, Department of Radiology, University of Virginia School of Medicine.

Journal of Visualized Experiments : Jove
|September 20, 2021
PubMed
Summary

Simplified methods for preparing targeted microbubbles, ultrasound contrast agents for molecular imaging, are presented. These streamlined protocols aim to accelerate clinical translation by improving efficiency and reproducibility.

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Area of Science:

  • Biomedical Engineering
  • Nanotechnology
  • Molecular Imaging

Background:

  • Microbubbles are crucial ultrasound contrast agents for molecular imaging.
  • Current preparation and targeting ligand attachment methods are complex and time-consuming.
  • Simplified protocols are needed for clinical translation of targeted microbubbles.

Purpose of the Study:

  • To provide a detailed, simplified procedure for targeted microbubble preparation, characterization, and testing.
  • To optimize protocols for clinical applicability.
  • To demonstrate the efficacy of two targeting systems: biotin-streptavidin and cyclic RGD peptide.

Main Methods:

  • Covalent coupling of targeting ligands to lipid anchors.
  • Microbubble preparation via amalgamation and size adjustment by flotation.
  • Characterization using electrozone sensing.
  • Binding assays in static and flow conditions.

Main Results:

  • Successful covalent attachment of targeting ligands.
  • Optimized microbubble size distribution.
  • Demonstrated targeted binding in vitro using biotin-streptavidin and RGD peptide systems.
  • Validated simplified preparation and testing protocols.

Conclusions:

  • The presented simplified methods facilitate the preparation and characterization of targeted microbubbles.
  • These optimized protocols are suitable for advancing targeted microbubble technology towards clinical applications.
  • The study provides a reproducible framework for developing targeted microbubbles for molecular imaging.