Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

DNA Agarose Gel Electrophoresis02:35

DNA Agarose Gel Electrophoresis

103.8K
Agarose gel electrophoresis is a laboratory technique commonly used to separate DNA fragments by size. However, it can also be used to isolate and purify DNA fragments using a gel extraction protocol.
Gel extraction follows five major steps: running gel electrophoresis to separate fragments, isolating the individual bands, extracting DNA from those bands, and removing the dye and salts from the extracted mixture to obtain pure DNA.
In cloning experiments, both the insert and vector DNA...
103.8K
Chemotaxis in E. coli01:27

Chemotaxis in E. coli

227
Chemotaxis in Escherichia coli is a sensory-driven motility mechanism that enables bacteria to navigate chemical gradients, moving toward beneficial environments while avoiding harmful conditions. This process relies on a signal transduction system integrating external chemical cues with flagellar motor control.Chemoreceptors and Signal DetectionE. coli detects chemical gradients through methyl-accepting chemotaxis proteins (MCPs), which are membrane-bound chemoreceptors that sense attractants...
227
Chemotaxis and Direction of Cell Migration01:21

Chemotaxis and Direction of Cell Migration

3.8K
Cells can detect chemical cues in their environment and reorganize the cytoskeleton to migrate toward them or away from them. This directional migration, called chemotaxis, is essential during embryogenesis and development, immune response, tissue repair and regeneration, and reproduction. These chemical cues can either attract or repel the cell's movement. For example, axon development is determined by a combination of chemoattractants and chemorepellents that direct the growing axon...
3.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A role for PaxB in regulating blebbing: experimental insights and theoretical perspectives from <i>Dictyostelium discoideum</i>.

Research square·2026
Same author

A mathematical model for bleb expansion clarifies a role for TalA in regulating blebbing.

bioRxiv : the preprint server for biology·2025
Same author

Bacterial metallothionein, PmtA, a novel stress protein found on the bacterial surface of <i>Pseudomonas aeruginosa</i> and involved in management of oxidative stress and phagocytosis.

mSphere·2024
Same author

Modeling the dynamics of actin and myosin during bleb stabilization.

bioRxiv : the preprint server for biology·2023
Same author

A role for myosin II clusters and membrane energy in cortex rupture for Dictyostelium discoideum.

PloS one·2022
Same author

A PKC that controls polyphosphate levels, pinocytosis and exocytosis, regulates stationary phase onset in Dictyostelium.

Journal of cell science·2022
Same journal

Mapping the 3D Chromosome Organization of a Biosynthetic Gene Cluster by Capture Hi-C (CHi-C).

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Mapping the 3D Chromosome Organization of Streptomyces by Hi-C.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

CUT&Tag Epigenomic Profiling of Biosynthetic Gene Clusters in Arabidopsis thaliana.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Rhizobium rhizogenes-Mediated Hairy Root Transformation Protocol for Lotus japonicus and Other Legumes.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Characterization of Bioactive Saponins from Sea Cucumbers.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Methods for Functional Validation of Terpenoid Metabolic Clusters in Nicotiana benthamiana and Aspergillus oryzae.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

Related Experiment Video

Updated: Oct 19, 2025

C. elegans Chemotaxis Assay
06:28

C. elegans Chemotaxis Assay

Published on: April 27, 2013

46.5K

Chemotaxis: Under Agarose Assay.

Derrick Brazill1,2, David A Knecht3

  • 1Department of Biological Sciences, Hunter College, New York, NY, USA. Brazill@GENECTR.HUNTER.CUNY.EDU.

Methods in Molecular Biology (Clifton, N.J.)
|September 20, 2021
PubMed
Summary
This summary is machine-generated.

Dictyostelium discoideum amoebae exhibit chemotaxis, a crucial cell-movement process. This study uses the under-agarose assay to explore conserved signaling pathways in Dictyostelium, aiding research into eukaryotic cell motility.

Keywords:
AmoebaChemotaxisDictyosteliumFolic acidcAMP

More Related Videos

Measurement of Cellular Chemotaxis with ECIS/Taxis
11:37

Measurement of Cellular Chemotaxis with ECIS/Taxis

Published on: April 1, 2012

15.0K
A Microfluidic Device for Quantifying Bacterial Chemotaxis in Stable Concentration Gradients
09:28

A Microfluidic Device for Quantifying Bacterial Chemotaxis in Stable Concentration Gradients

Published on: April 19, 2010

12.3K

Related Experiment Videos

Last Updated: Oct 19, 2025

C. elegans Chemotaxis Assay
06:28

C. elegans Chemotaxis Assay

Published on: April 27, 2013

46.5K
Measurement of Cellular Chemotaxis with ECIS/Taxis
11:37

Measurement of Cellular Chemotaxis with ECIS/Taxis

Published on: April 1, 2012

15.0K
A Microfluidic Device for Quantifying Bacterial Chemotaxis in Stable Concentration Gradients
09:28

A Microfluidic Device for Quantifying Bacterial Chemotaxis in Stable Concentration Gradients

Published on: April 19, 2010

12.3K

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Dictyostelium discoideum is a unicellular eukaryote widely used to study chemotaxis.
  • Chemotaxis in Dictyostelium involves distinct molecular mechanisms under vegetative (pterin-responsive) and starved (cAMP-responsive) conditions.
  • The organism's genetic tractability and conserved signaling pathways make it an excellent model for human cell research.

Purpose of the Study:

  • To detail the under-agarose chemotaxis assay for analyzing directional sensing and motility in Dictyostelium.
  • To investigate the conserved molecular signaling pathways governing eukaryotic chemotaxis.
  • To leverage Dictyostelium as a model system for understanding mammalian cell behavior.

Main Methods:

  • Utilizing the under-agarose chemotaxis assay to observe and quantify cell movement in response to chemoattractants.
  • Employing genetic and molecular techniques to dissect signaling pathways in Dictyostelium discoideum.
  • Comparing signaling mechanisms in Dictyostelium with those in mammalian cells.

Main Results:

  • The under-agarose assay effectively visualizes chemotactic responses in Dictyostelium.
  • Key signaling pathways controlling directional sensing and motility were elucidated.
  • Identified conserved elements between Dictyostelium and mammalian chemotaxis.

Conclusions:

  • Dictyostelium discoideum serves as a powerful model for dissecting conserved eukaryotic chemotaxis pathways.
  • The under-agarose assay is a valuable tool for studying cell motility and signal transduction.
  • Findings contribute to understanding fundamental biological processes relevant to human health.