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Updated: Oct 19, 2025

A Time-Efficient Fluorescence Spectroscopy-Based Assay for Evaluating Actin Polymerization Status in Rodent and Human Brain Tissues
Published on: June 3, 2021
Functional Analysis of Actin-Binding Proteins in the Central Nervous System of Drosophila
Qi He1, Christopher Roblodowski2
1Department of Biology, Brooklyn College of the City University of New York, Brooklyn, NY, USA. QHe@brooklyn.cuny.edu.
Abstract:
Using Drosophila actin-binding protein Dunc-115 as an example, this chapter describes a MARCM (mosaic analysis with a repressible cell marker)-based method for analyzing cytoskeletal components for their functions in the nervous system. Following a concise description about the principle, a step-by-step protocol is provided for generating the needed stocks and for histological analysis. Additional details and explanations have been given in the accompanying notes. Together, this should form a practical and sufficient recipe for performing at the single cell level loss-of-function and gain-of-function analyses of proteins associated with the cytoskeleton.
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