Related Experiment Video

Updated: Oct 19, 2025

Determining Intestinal Permeability Using Lucifer Yellow in an Apical-Out Enteroid Model
09:48

Determining Intestinal Permeability Using Lucifer Yellow in an Apical-Out Enteroid Model

Published on: July 27, 2022

3.6K

Assessment of small-intestine permeability in healthy Nigerian children is altered by urinary volume and voiding

Ibukun Afolami1,2, Folake Olukemi Samuel1, Martin Mwangi2

  • 1Department of Human Nutrition, University of Ibadan, Ibadan, Nigeria.

Plos One
|September 20, 2021
PubMed

Insights

Urinary volume significantly impacts small intestine permeability tests in children. Higher urinary volume correlates with altered sugar recovery, suggesting volume is a key factor in test interpretation.

Area of Science:

  • Pediatric Gastroenterology
  • Clinical Nutrition
  • Biomedical Research

Background:

  • Small intestine permeability is a crucial indicator of gut health.
  • Multi-sugar tests are commonly used to assess intestinal permeability.
  • Factors influencing test accuracy, such as urinary volume, require investigation.

Purpose of the Study:

  • To investigate the effect of voided urinary volume on small intestine permeability ratios in healthy children.
  • To determine if urinary volume influences the interpretation of multi-sugar permeability tests.

Main Methods:

  • Assessed small intestine permeability in 155 healthy Nigerian children (3-5 years) using a multi-sugar test (lactulose, sucrose, mannitol, rhamnose).
  • Categorized children into low (LV) and high (HV) urinary volume groups based on percentile rankings.
  • Compared sugar recovery ratios and permeability indices between LV and HV groups, and between voiding (VC) and non-voiding (NVC) children.

Main Results:

  • Higher urinary volume (HV) was associated with significantly increased mannitol and rhamnose recovery compared to low urinary volume (LV).
  • Lactulose recovery was lower in HV children, though not statistically significant (p=0.071).
  • Lactulose-mannitol, lactulose-rhamnose, and sucrose-rhamnose ratios were significantly higher in the LV group, indicating altered permeability.

Conclusions:

  • Total urinary volume is a significant variable affecting small intestine permeability measurements in multi-sugar tests.
  • Voiding status before testing may independently influence specific sugar recovery and permeability ratios.
  • Recommendations for conducting multi-sugar small intestine permeability tests should consider and account for variations in urinary volume.
Abstract

More Related Videos

Functional Assessment of Intestinal Permeability and Neutrophil Transepithelial Migration in Mice using a Standardized Intestinal Loop Model09:24

Functional Assessment of Intestinal Permeability and Neutrophil Transepithelial Migration in Mice using a Standardized Intestinal Loop Model

Published on: February 11, 2021

7.4K
Functional Assessment of Intestinal Tight Junction Barrier and Ion Permeability in Native Tissue by Ussing Chamber Technique06:43

Functional Assessment of Intestinal Tight Junction Barrier and Ion Permeability in Native Tissue by Ussing Chamber Technique

Published on: May 26, 2021

5.9K

Related Concept Videos

Urodynamic Studies: Uroflowmetry01:19

Urodynamic Studies: Uroflowmetry

Uroflowmetry is a non-invasive urodynamic test designed to measure various aspects of urination, including volume, flow rate, and the time to void. This test is crucial for diagnosing and assessing conditions such as bladder outlet obstruction, bladder dysfunction, incomplete bladder emptying, incontinence, and urinary tract blockages caused by benign prostatic hyperplasia (BPH) and urethral strictures.Pre-Test Instructions:Before a uroflowmetry test, patients are typically advised to drink...
998
Pharmacokinetics in Pediatric Patients: Overview and Drug Absorption01:23

Pharmacokinetics in Pediatric Patients: Overview and Drug Absorption

Understanding the physiological differences in the pediatric population is crucial for effective pharmacotherapy. Neonates, infants, and children exhibit significant variations in gastric pH, gastric emptying time, intestinal transit time, and biliary function. These variations profoundly affect oral drug absorption, necessitating a nuanced approach to pediatric dosing.Neonates present with a unique physiological profile, having a gastric pH greater than 4 and faster and more irregular gastric...
31
Physiology of the Genitourinary System III: Urine Concentration and Dilution01:20

Physiology of the Genitourinary System III: Urine Concentration and Dilution

The kidneys concentrate or dilute urine to maintain water and electrolyte balance. Nephrons, particularly the loop of Henle, play a crucial role in this process through the countercurrent multiplication system. This system establishes a high osmolarity in the renal medulla, which is essential for water reabsorption. In the loop of Henle’s descending limb, water is reabsorbed into the surrounding medulla due to its permeability to water. In contrast, the ascending limb actively transports...
130
Assessment of the Gastrointestinal System II: Health Perception Pattern01:29

Assessment of the Gastrointestinal System II: Health Perception Pattern

Assessing the gastrointestinal (GI) system is a complex process that begins with collecting subjective data. This data, collected through patient interviews, provides crucial insights into the patient's health history, perception patterns, and lifestyle habits, all contributing significantly to GI health.
Health Perception Patterns
Health perception patterns offer valuable insights into a patient's lifestyle habits and how they may impact their GI health. These patterns include:
201
Methods for Studying Drug Absorption: In situ01:09

Methods for Studying Drug Absorption: In situ

In situ experiments, such as the Doluisio method and Single-Pass Perfusion technique, provide critical insights into drug uptake by simulating in vivo conditions for drug absorption.
The Doluisio method involves perfusing a prepared segment of a rat's small intestine with a solution of radiolabeled drug and a non-absorbable marker. This helps to differentiate between absorbed and non-absorbed drug concentrations. The intestinal segment is connected at both ends using tubing and syringes,...
403
Assessment of the Rectum and Anus01:25

Assessment of the Rectum and Anus

Evaluating the rectum and anus plays a crucial role in conducting a thorough physical examination of the gastrointestinal system. Although it may be uncomfortable and often embarrassing for the patient, it holds immense diagnostic value, particularly in detecting gastrointestinal diseases and abnormalities. This guide will explain how to perform this assessment using inspection and palpation methods.
Rectal Inspection
Begin by inspecting the perianal and anal areas for color, texture, rashes,...
514