Acclimation of an algal consortium to sequester nutrients from anaerobic digestate

The objective of this research was to investigate the growth, community composition, and digestate treatment performance of a local algae consortium that was adapted to bacteria-pretreated digestate. The approach was to subculture a local consortium on pretreated dairy manure digestate and then municipal wastewater sludge digestate, allowing the community to adapt before assessing its performance. The adapted consortium was then tested for growth and nutrient removal performance on the digestates and compared to the model organism, Chlorella sorokiniana. Dramatic restructuring of the consortium took place when subcultured on the digestates with Scenedesmaceae and Chlorellaceae almost completely replacing Euglena. The consortium was consistently less productive than C. sorokiniana (184 vs. 248 mg/L/d in dairy digestate and 32 vs. 48 mg/L/d in municipal digestate, P < 0.01). Pretreatment increased growth by 81% and 500% for C. sorokiniana and the consortium, respectively, in dairy digestate (P < 0.01), and allowed for algal growth in municipal digestate.