Dual cathode configuration and headspace gas recirculation for enhancing microbial electrosynthesis using Sporomusa...

High-rate production of acetate and other value-added products from the reduction of CO₂ in microbial electrosynthesis (MES) using acetogens can be achieved with high reducing power where H₂ appears as a key electron mediator. H₂ evolution using metal cathodes can enhance the availability of H₂ to support high-rate microbial reduction of CO₂. Due to the low solubility of H₂, the availability of H₂ remains limited to the bacteria. In this study, we investigated the performances of Sporomusa ovata for CO₂ reduction when dual cathodes were used together in an MES, one was regular carbon cathode, and the other was a titanium mesh that allows higher hydrogen evolution. The dual cathode configuration was investigated in two sets of MES, one set had the usual S. ovata inoculated graphite rod, and another set had a synthetic biofilm-imprinted carbon cloth. Additionally, the headspace gas in MES was recirculated to increase the H₂ availability to the bacteria in suspension. High-rate CO₂ reduction was observed at -0.9 V vs Ag/AgCl with dual cathode configuration as compared to single cathodes. High titers of acetate (up to ∼11 g/L) with maximum instantaneous rates of 0.68-0.7 g/L/d at -0.9 V vs Ag/AgCl were observed, which are higher than the production rates reported in the literatures for S. ovata using MES with surface modified cathodes. A high H₂ availability supported the high-rate acetate production from CO₂ with diminished electricity input.